Abstract
Identification of several distinct cell surface molecules expressed by human T lymphocytes has been made by the use of murine monoclonal antibodies (MoAb) developed against lymphoid cells [2, 3, 5, 13]. The purpose of the present study was to determine the number of unique T lymphocyte antigens which could be identified using the Workshop T lymphocyte MoAb. The serological component of the investigation involved the classification of the MoAb into groups based on their particular serological patterns of reactivity with the target cell panel. The results were then compared with data about the distinct cell surface molecules which could be detected by immunoprecipitation analysis using these antibodies. In the process we wanted to design a limited target cell panel which could be used in the future to serologically distinguish each of these T lymphocyte antigens. The study demonstrated that distinct serological patterns of each of the T lymphocyte antigens could be obtained, and the results of immunoprecipitation of the cell surface antigens with the MoAb confirmed the serological classification.
This study was supported by grants from the United States Public Health Service, NIH, NCI, CA-22507, CA-23766, and CA-33050, and from the XOMA Corporation
We gratefully acknowledge Bernadette Kienzle for her technical assistance and Seth Horowitz for preparation of the manuscript
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© 1984 Springer-Verlag Berlin Heidelberg
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Horibe, K., Knowles, R.W., Naito, K., Morishima, Y., Dupont, B. (1984). Analysis of T Lymphocyte Antibody Specificities: Comparison of Serology with Immunoprecipitation Patterns. In: Bernard, A., Boumsell, L., Dausset, J., Milstein, C., Schlossman, S.F. (eds) Leucocyte Typing. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68857-7_6
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DOI: https://doi.org/10.1007/978-3-642-68857-7_6
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