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Analysis of B Cell-CALLA and Monocyte-Granulocyte Protocol Reagents Using Immunofluorescence, Tissue Sections (PAP) and Molecular Weight Determination

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Leucocyte Typing

Abstract

The 92 monoclonal antibodies from the B cell-CALLA (B1–B34, BC− and BC+) and monocyte-granulocyte (M1–M54, MC− and MC+) protocols were analysed by indirect immunofluorescence using flow cytofluorometry by a modified immunoperoxidase (PAP) technique [1] and by immunoprecipitation [2] (to determine molecular weight).

This work was supported by a grant from the Research Committee, Cancer Institute, Melbourne

The authors wish to thank L. Bradley for excellent technical assistance and G. White for typing the manuscript

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References

  1. Hancock W, Becker GJ, Atkins RC (1982) A comparison of fixatives and immunohistochemical techniques for use with monoclonal antibodies to cell surface antigens. Am J Clin Pathol 78: 825–831

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  2. De Kretser TA, Crumpton MC, Bodmer JG, Bodmer WF (1982) Demonstration of two distinct light chains in HLA-DR-associated antigens by two-dimensional gel electrophoresis. Eur J Immunol 12: 214–221

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  3. Bradley TR, Pilkington GR, Garson M, Hodgson GS, Kraft N (1982) Cell lines derived from a human myelomonocytic leukaemia. Br J Haematol 51: 595–604

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© 1984 Springer-Verlag Berlin Heidelberg

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Pilkington, G.R. et al. (1984). Analysis of B Cell-CALLA and Monocyte-Granulocyte Protocol Reagents Using Immunofluorescence, Tissue Sections (PAP) and Molecular Weight Determination. In: Bernard, A., Boumsell, L., Dausset, J., Milstein, C., Schlossman, S.F. (eds) Leucocyte Typing. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68857-7_44

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  • DOI: https://doi.org/10.1007/978-3-642-68857-7_44

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-68859-1

  • Online ISBN: 978-3-642-68857-7

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