Abstract
We have previously reported the value of immunohistological screening on tissue sections, both as a rapid method of analysing the reactivity patterns of monoclonal antibodies against individual antigens and as a means of identifying new monoclonal antibodies which would be difficult to detect by conventional screening methods [4]. For the First International Workshop on Human Leucocyte Differentiation Antigens we have screened all the antibodies received in each of the three panels (T, B and macrophage) on a variety of acetone-fixed cryostat tissue sections to establish their pattern of reactivity, to detect any tissue cross-reactions, and to compare the intensity of reaction between similar antibodies. All the antibodies were also screened on paraffin-embedded sections of lymphoid tissue in order to evaluate their possible role in routine histopathological diagnosis.
This work was supported by the Cancer Research Campaign, the Wellcome Trust and the Leukaemia Research Fund
We thank Roy Holton for his photographic assistance and Tina Chisnall for typing the manuscript
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© 1984 Springer-Verlag Berlin Heidelberg
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Pulford, K.A.F., Knight, P.M., Gatter, K.C., Mason, D.Y. (1984). Immunocytochemical Characterization of Monoclonal Anti-Leucocyte Antibodies. In: Bernard, A., Boumsell, L., Dausset, J., Milstein, C., Schlossman, S.F. (eds) Leucocyte Typing. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68857-7_40
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DOI: https://doi.org/10.1007/978-3-642-68857-7_40
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