Abstract
Recently many monoclonal antibodies (MoAb) which define new granulocyte- and/or monocyte-specific antigens have been described. These antibodies have been applied to the study of myeloid and monocyte differentiation [8, 9, 11], as well as to the typing of the cells of patients with leukemia [5, 10]. In this report we describe three new monoclonal antibodies, two reacting with granulocytes and one reacting with granulocytes, monocytes, and a population of non-T lymphocytes. The molecular identity of the antigens recognized by the antibodies and the expression of the antigens in hybrids derived from fusion of human and mouse myeloid cells was studied. They were also compared with monoclonal antibodies against granulocytes from other laboratories.
This work was supported by the Netherlands Cancer Society. (Koningin Wilhelmina Fonds)
We thank Dr. W. Knapp, Dr. J.T. Kemshead and Dr. H. Zola for providing their monoclonal antibodies, Mrs M.J. E. Bos, Mr. F. J. Visser and Mr. A. J. van Agthoven for their technical contribution
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Astaldi GCB, Janssen MC, Landsdorp PM, Willems C, Zeijlemaker WP, Oosterhof F (1980) Human endothelial culture supernatant (HECS): a growth factor for hybridomas. J Immunol 125: 1411–1414
Barnstable CJ, Bodmer WF, Brown G, Gafré G, Milstein C, Williams AF, Ziegler A (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell 14: 9–20
Borst J, Prendiville MA, Terhorst C (1982) Complexicity of the human T-lymphocyte-specific cell surface antigen T3. J Immunol 128: 1560–1565
Goodfellow PM, Banting G, Wiles MV, Tunnacliffe A, Parker M, Solomon E, Dalchau R, Fabre JW (1982) The gene, MIC4, which controls expression of the antigen defined by monoclonal antibody F10.44.2. is on human chromosome 11. Eur J Immunol 12: 659–663
Griffin JD, Ritz J, Nadler LM, Schlossmann SF (1981) Expression of myeloid differentiation antigens on normal and malignant myeloid cells. J Clin Invest 68: 932–941
Kemshead JT, Bicknell D, Greaves MF (1981) A monoclonal antibody detecting an antigen shared by neural and granulocyte cells. Pediatr Res 15: 1281–1286
Landsdorp PM, Astaldi GCB, Oosterhof F, Janssen M, Zeijlemaker WP (1980) Immunoperoxidase procedure to detect monoclonal antibodies against cell surface antigens. Quantification of binding and staining of individual cells. J Immunol Methods 39: 393–405
Majdic O, Liszka K, Lutz D, Knapp W (1981) Myeloid differentiation antigen defined by a monoclonal antibody. Blood 58: 1127–1133
Perussia B, Trinchieri G, Lebman D, Jankiewicz J, Lange B, Rovera G (1982) Monoclonal antibodies that detect differentiation surface antigens on human myelomonocytic cells. Blood 59: 382–391
van der Reijden HG, van Rehnen DJ, Lansdorp PM, van ‘t Veer MB, Langenhuijsen MMAC, Engelfriet CP, von dem Borne AEG (1983) A comparison of surface markers and FAB classification in acute myeloid leukaemia. Blood 61: 443–448
Zola H, McNamara P, Thomas M, Smart IJ, Bradley J (1981) The preparation and properties of monoclonal antibodies against human grnulocyte membrane antigens. Br J Haematol 48: 481–490
Geurts van Kessel AHM, Tetteroo PAT, von dem Borne AEGK, Hagemeijer A, Bootsma D (1983) Expression of human myeloid-associated surface antigens in human-mouse myeloid cell hybrids. Proc Natl Acad Sci USA 80: (in press)
Breard J, Reinherz EL, Kung PC, Goldstein G, Schlossmann SF (1980) A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol 124: 1943–1948
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Tetteroo, P.A.T., Lansdorp, P.M., Geurts van Kessel, A.H.M., Hagemeijer, A., von dem Borne, A.E.G.K. (1984). Serological and Biochemical Characterization of Human Myeloid-Associated Antigens and Their Expression in Human-Mouse Cell Hybrids. In: Bernard, A., Boumsell, L., Dausset, J., Milstein, C., Schlossman, S.F. (eds) Leucocyte Typing. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68857-7_34
Download citation
DOI: https://doi.org/10.1007/978-3-642-68857-7_34
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-68859-1
Online ISBN: 978-3-642-68857-7
eBook Packages: Springer Book Archive