Membrane Antigens of Human Myeloid Cells Defined by Monoclonal Antibodies

  • J. R. McKolanis
  • M. J. Borowitz
  • F. L. Tuck
  • R. S. Metzgar
Conference paper


The development of technology to produce monoclonal antibodies for the immunological detection of cell membrane antigens has been extensively applied to the identification of human lymphocyte cell surface antigens. This approach has led to the isolation of functional subsets of lymphoid cells [3] and the phenotypic resolution of discrete stages of lymphocyte differentiation which are often duplicated on leukemic cells [6]. More recently, monoclonal antibodies reactive with mature myeloid and monocytic type cells of normal donors have been described [2, 8, 10, 15]. These antibodies detect antigens which are not on lymphoid cells but show different specificities for nonlymphoid cells. Some monoclonal antibodies react only with monocytes or granulocytes [10, 15], while other antibodies react with both peripheral blood monocytes and granulocytes [8]. Most of these antibodies react with myeloid precursor cells in the bone marrow or cells undergoing early myeloid differentiation. These antigens may then appear at discrete stages of myeloid maturation or on cells from some patients with myeloid and/or monocytic leukemia.


Peripheral Blood Monocyte Monocytic Leukemia Cell Sorter Analysis Acute Myelogenous Leukemia Cell Nonlymphoid Cell 
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  1. 1.
    Anderson JK, Metzgar RS (1978) Detection and partial characterization of human T and B lymphocyte membrane antigens with antisera to HSB and SB cell lines. J Immunol 120: 262–271PubMedGoogle Scholar
  2. 2.
    Bernstein ID, Andrews RG, Cohen SF, McMaster BE (1982) Normal and malignant human myelocytic and monocytic cells identified by monoclonal antibodies. J Immunol 128: 876–881PubMedGoogle Scholar
  3. 3.
    Engleman EG, Benike CJ, Glickman E, Evans RL (1981) Antibody to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med 154: 193–198PubMedCrossRefGoogle Scholar
  4. 4.
    Ferrante A, Tong YH (1978) A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the Hypaque-Ficoll technique. J Immunol Meth 24: 389CrossRefGoogle Scholar
  5. 5.
    Fischer DG, Hubbard WJ, Koren HS (1981) Tumor cell killing by freshly isolated peripheral blood monocytes. Cell Immunol 58: 426–430PubMedCrossRefGoogle Scholar
  6. 6.
    Foon KA, Schroff RW, Gale RP (1982) Surface markers on leukemia and lymphoma cells: recent advances. Blood 60: 1–9PubMedGoogle Scholar
  7. 7.
    Galfre G, Howe SC, Milstein C, Butcher GW, Howard JC (1977) Antibodies to major histocompatibility antigen produced by hybrid cell lines. Nature 266: 550–552PubMedCrossRefGoogle Scholar
  8. 8.
    Griffin JD, Ritz J, Nadler LM, Schlossman SF (1981) Expression of myeloid differentiation antigens on normal and malignant myeloid cells. J Clin Invest 68: 932–941PubMedCrossRefGoogle Scholar
  9. 9.
    Hoffman RA, Hansen WP (1981) Immunofluorescent analysis of Blood cells by flow cytometry. Int J Immunopharmacol 3: 249–254PubMedCrossRefGoogle Scholar
  10. 10.
    Linker-Israeli M, Billing RJ, Foon KA, Terasaki PI (1981) Monoclonal antibodies reactive with acute myelogenous leukemia cells. J Immunol 127: 2473–2477PubMedGoogle Scholar
  11. 11.
    Metzgar RS, Borowitz MJ, Jones NH, Dowell BL (1981) Distribution of common acute lymphoblastic leukemia antigen in nonhematopoietic tissues. J Exp Med 154: 1249–1254PubMedCrossRefGoogle Scholar
  12. 12.
    Metzgar RS, Gaillard MT, Levine SJ, Tuck FL, Bossen E, Borowitz MJ (1982) Antigens of human pancreatic adenocarcinoma cells defined by murine monoclonal antibodies. Cancer Res 42: 601–608PubMedGoogle Scholar
  13. 13.
    Mohanakumar T, Metzgar RS, Miller DS (1974) Human leukemia cell antigens: Serologic characterization with xenoantisera. J Natl Cancer Inst 52: 1435–1444PubMedGoogle Scholar
  14. 14.
    Moore MAS, Broxmeyer HE, Sheridan APC, Myers PA, Jacobson N, Winchester RJ (1980) Continuous human bone marrow culture: la antigen characterization of probable pluripotential stem cells. Blood 55: 682–690PubMedGoogle Scholar
  15. 15.
    Perussia B, Trinchieri G, Lebman D, Jankiewicz J, Lange B, Rovera G (1982) Monoclonal antibodies that detect different surface antigens on human myelomonocytic cells. Blood 59: 382–392PubMedGoogle Scholar
  16. 16.
    Winchester RJ, Kunkel HG (1979) The human la system. Adv Immunol 28: 221–291PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • J. R. McKolanis
  • M. J. Borowitz
  • F. L. Tuck
  • R. S. Metzgar

There are no affiliations available

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