Abstract
The events leading to the stimulation of B lymphocytes are not yet well characterized. In addition to the specific role of surface immunoglobulin, a variety of receptors and antigenic molecules, of cellular interactions, and of proteolytic reactions has been recognized to participate in the regulation of B lymphocyte activity. The present studies were initiated to learn about quantitative and qualitative changes of surface structures during B lymphocyte differentiation and plasma cell formation.
Supported by Stiftung Volkswagenwerk
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bross KJ, Pangalis GA, Staatz CG, Blume KG (1978) Demonstration of cell surface antigens and their antibodies by the peroxidase-anti-peroxidase method. Transplantation 25: 331
Douillard JY, Hoffman T, Herberman RB (1980) Enzyme-linked immunosorbent assay for screening monoclonal antibody production: Use of intact cells as antigens. J Immunol Methods 39: 309
English D, Anderson BR (1974) Single-step separation of red blood cells, granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque. J Immunol Methods 5: 249
Galfré G, Howe SC, Milstein C, Butcher GW, Howard JC (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266: 550
Kumagai K, Itoh K, Hinuma S, Tada M (1976) Pretreatment of plastic petri dishes with fetal calf serum. A simple method for macrophage isolation. J Immunol Methods 29: 17
Schauenstein K, Wick G (1976) The micro-membrane-fluorescence test: A new semiautomated technique based on the microtiter system. J Immunol Methods 10: 143
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase—anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18: 315
Stocker JW, Heusser CH (1979) Methods for binding cells to plastic: Application to a solid-phase radioimmunoassay for cell-surface antigens. J Immunol Methods 26: 87
Wahl SM, Rosenstreich DL, Oppenheim JJ (1976) Separation of human lymphocytes by E-rosette sedimentation. In: Bloom BR, David JR (eds) In vitro methods in cell-mediated and tumor immunity. Academic, New York p 231
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Morich, FJ., Momburg, F., Hartmann, KU. (1984). Surface Antigens of Subpopulations of Mitogen-Stimulated Human Lymphocytes. In: Bernard, A., Boumsell, L., Dausset, J., Milstein, C., Schlossman, S.F. (eds) Leucocyte Typing. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68857-7_27
Download citation
DOI: https://doi.org/10.1007/978-3-642-68857-7_27
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-68859-1
Online ISBN: 978-3-642-68857-7
eBook Packages: Springer Book Archive