Characterization of Functional Human T Cell Subsets by Monoclonal Antibodies
Monoclonal antibodies (MoAb) directed to differentiation antigens on human leucocytes have proved to be extremely valuable tools for the dissection of the immune system. It has become clear that the expression of unique surface determinants on certain immunoregulatory cells apparently correlates with function in the regulatory network of cell interactions. In our attempt to raise MoAb against differentiation antigens on mature T lymphocytes, mice were immunized with homogeneous leukemic cell populations obtained from patients suffering from chronic lymphocytic leukemia (CLL) of T type. This strategy was based on the idea that the leukemic cells of a T-CLL might be the clonally expanded, well-differentiated malignant counterpart of a normal T cell phenotype representing a distinct functional subset.
KeywordsChronic Lymphocytic Leukemia Cell Subset Peripheral Blood Lymphocyte Plaque Form Cell Lytic Unit
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