Abstract
Monoclonal antibodies (MoAb) directed to differentiation antigens on human leucocytes have proved to be extremely valuable tools for the dissection of the immune system. It has become clear that the expression of unique surface determinants on certain immunoregulatory cells apparently correlates with function in the regulatory network of cell interactions. In our attempt to raise MoAb against differentiation antigens on mature T lymphocytes, mice were immunized with homogeneous leukemic cell populations obtained from patients suffering from chronic lymphocytic leukemia (CLL) of T type. This strategy was based on the idea that the leukemic cells of a T-CLL might be the clonally expanded, well-differentiated malignant counterpart of a normal T cell phenotype representing a distinct functional subset.
Part of this work was supported by the Deutsche Forschungsgemeinschaft, Bonn, Sonderforschungsbereich 37 and by the Curt-Bohnewand-Fonds, University of Munich
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References
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497
Feucht HE, Hadam MR, Frank F, Riethmüller G (1980) Efficient separation of human T lymphocytes from venous blood using PUP-coated colloidal silica particles ( Percoll ). J Immunol Methods 38: 43–51
Lohmeyer J, Rieber P, Feucht H, Johnson J, Hadam M, Riethmüller G (1981) A subset of human natural killer cells isolated and characterized by monoclonal antibodies. Eur J Immunol 11: 997–1101
Rieber P, Lohmeyer J, Schendel DJ, Riethmüller G (1981) Human T cell differentiation antigens characterizing a cytotoxic/suppressor T cell subset. Hybridoma 1: 59–69
Schendel DJ, Wank R, Bonnard GD (1980) Genetic specificity of primary and secondary proliferative and cytotoxic responses of human lymphocytes grown in continued culture. Scand J Immunol 11: 99–107
Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1979) Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci USA 76: 4061–4065
Evans RL, Wall DW, Platsoucas CD, Siegal FP, Fikrig SM, Testa CM, Good RA (1981) Thymus-dependent membrane antigens in man: Inhibition of cell-mediated lympholysis by monoclonal antibodies to the TH2 antigen. Proc Natl Acad Sci USA 78: 544–548
Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1980) A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2. J Immunol 124: 1301–1307
Hoffman-Fezer G, Lohmeyer J, Doxiadis I, Stünkel K, Rieber EP, Kummer U, Eulitz M, Thierfelder S (1982) Monoclonal antibodies against T cell antigens studied by immunohistochemistry. Blut 44: 275–288
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© 1984 Springer-Verlag Berlin Heidelberg
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Rieber, P. et al. (1984). Characterization of Functional Human T Cell Subsets by Monoclonal Antibodies. In: Bernard, A., Boumsell, L., Dausset, J., Milstein, C., Schlossman, S.F. (eds) Leucocyte Typing. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68857-7_18
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DOI: https://doi.org/10.1007/978-3-642-68857-7_18
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