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Phenomenon of Rosette Formation of Human T Cells Analyzed with Monoclonal Antibodies. The Same Mechanism of Interaction Accounts for Rosette Formation with Erythrocytes from Many Species, Including Autologous Erythrocytes

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Leucocyte Typing

Abstract

Spontaneous rosette formation with sheep erythrocytes has been used for many years to identify and enumerate human T cells [1-3]. The molecule of the T cell surface responsible for this fortuitous interaction was clearly identified after the development of the monoclonal antibody (MAb) 9.6 [4], and subsequently, LeuS, OKT11, and anti-T11 [5–8]. All these reagents recognize the same, or closely related, epitopes. In our laboratory, we obtained another antibody termed anti-D66, which also reacts with this molecule but defines another epitope [9], since no cross inhibition of fixation occurs between anti-D66 and the other reagents. We showed that anti-D66 has a strikingly different blocking property to 9.6 and anti-T11 in rosette formation with sheep erythrocytes (E); it can inhibit rosette formation between sheep E and T cells only when sheep E are not pretreated with 2-amino-ethylisothiouronium bromide (AET) or neuraminidase, a procedure known to increase the stability of their interactions with T cells [10, 11]. The D66 epitope appears to determine the temperature dependency of the rosette stability of a given T cell population with sheep E, since only populations with high D66 surface density can form rosettes stable at 37 °C with sheep E [9]. In addition, unlike epitopes defined by 9.6, many D66 epitopes are masked by sialic acid.

This work was supported by Association pour la Recherche sur le Cancer (Villejuif), IGR 81 D 8, INSERM 812001 and 129003

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References

  1. Lay WH, Mendes NF, Bianco C, Nussenzweig V (1971) Binding of sheep red blood cells to a large population of human lymphocytes. Nature 230: 531

    Article  PubMed  CAS  Google Scholar 

  2. Wybran J, Carr MC, Fudenberg HH (1972) The human rosette forming cell as a marker of a population of thymus-derived cells. J Clin Invest 51: 2537

    Article  PubMed  CAS  Google Scholar 

  3. Jondal M, Holm G, Wigzell H (1972) Surface markers on human T and B lymphocytes:1. A large population of lymphocytes forming non-immune rosettes with sheep red blood cells. J Exp Med 136: 207

    Google Scholar 

  4. Kamoun M, Martin PJ, Hansen JA, Brown MA, Siadak AW, Nowinski RC (1981) Identification of a human T lymphocyte surface protein associated with the E-rosette receptor. J Exp Med 153: 207

    Article  PubMed  CAS  Google Scholar 

  5. Meuer SC, Reinherz EL, Schlossman SF (1982) Clonal analysis of human cytotoxic T lymphocytes T4+ and T8+. Effector cells recognize products of different MHC regions. Proc Natl Acad Sci USA 79: 4390

    Google Scholar 

  6. Howard FD, Ledbetter JA, Wong J, BieberCP, Stinson EB, Herzenberg LA (1981) A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation. J Immunol 126: 2117

    Google Scholar 

  7. Van Wauwe J, Goossens J, Decock W, Kung P, Goldstein G (1981) Suppression of human T cell mitogenesis and E-rosette formation by the monoclonal antibody OKT11 A. Immunolgy 44: 865

    Google Scholar 

  8. Verbi W, Greaves MF, Schneider C, Koubek K, Janossy G, Stein H, Kung P, Goldstein G (1982) Monoclonal antibodies OKT11 and OKT11 A have pan-T reactivity and block sheep erythrocyte “receptors”. Eur J Immunol 12: 81

    Article  PubMed  CAS  Google Scholar 

  9. Bernard A, Gelin C, Raynal B, Pham D, Gosse C, Boumsell L (1982) Phenomenon of human T cells rosetting with sheep erythrocytes analyzed with monoclonal antibodies. “Modulation” of a partially hidden epitope determining the conditions of interaction between T cells and erythrocytes. J Exp Med 155: 1317

    Article  PubMed  CAS  Google Scholar 

  10. Bentwich Z, Douglas SD, Skutelsky E, Kunkel HG (1973) Sheep red cell binding to human lymphocytes treated with neuraminidase; enhancement of T cell binding and identification of a subpopulation of B cells. J Exp Med 137: 1532

    Article  PubMed  CAS  Google Scholar 

  11. Weiner MS, Bianco C, Nussenzweig V (1973) Enhanced binding of neuraminidase treated sheep erythrocytes to human T lymphocytes. Blood 42: 939

    PubMed  CAS  Google Scholar 

  12. Yu DTY, Dale RP (1977) Human Iymphocytes subpopulations: rabbit red blood cell rosettes. J Immunol Methods 16: 283

    Article  PubMed  CAS  Google Scholar 

  13. Lohrmann HP, Novikous L (1974) Rosette formation between human T-lymphocytes and un-sensitized rhesus monkey erythrocytes. Clin Immunol Immunopathol 3: 99

    Article  CAS  Google Scholar 

  14. Baxley G, Bishop GB, Cooper AG, Wortis HH (1973) Rosetting of human red blood cells to thymocytes and thymus-derived cells. Clin Exp Immunol 15: 385

    PubMed  CAS  Google Scholar 

  15. Gluckman JC, Montambault P (1975) Spontaneous autorosette forming cells in man. A marker for a subset population of T lymphocytes. Clin Exp Immunol 22: 302

    Google Scholar 

  16. Charreire J, Bach JF (1974) Self and not self. Lancet 2: 229

    Google Scholar 

  17. Kolb H (1977) A receptor for “self” on lymphocytes. Immunology 33: 859

    PubMed  CAS  Google Scholar 

  18. Boumsell L, Coppin H, Pham D, Raynal B, Lemerle J, Dausset J, Bernard A (1980) An antigen shared by a human T cell subset and B-CLL cells: distribution on normal and malignant lymphoid cells. J Exp Med 152: 229

    Article  PubMed  CAS  Google Scholar 

  19. Boumsell L, Bernard A (1981) Surface antigens on malignant T cells. In: Knapp W (ed) Leukemia marker conference. Springer, Berlin Heidelberg New York, pp 129–141

    Google Scholar 

  20. Bernard A, Boumsell L, Reinherz EL, Nadler LM, Ritz J, Coppin H, Richard Y, Valensi F, Flandrin G, Dausset J, Lemerle J, Schlossman SF (1981) Cell surface characterization of malignant T cells from lymphoblastic lymphoma using monoclonal antibodies. Evidence for phenotypic differences between malignant T cells from patients with acute lymphoblastic leukemia and lymphoblastic lymphoma. Blood 57: 1105

    Google Scholar 

  21. Boumsell L, Bernard A (1980) High efficiency of Biozzi’s high responder mouse strain in the generation of antibody secreting hybridomas. J Immunol Methods 38: 225

    Article  PubMed  CAS  Google Scholar 

  22. Boumsell L, Bernard A, Coppin H, Richard Y, Penit C, Rouget P, Lemerle J, Dausset J (1979) Human T cell differentiation antigens and correlation of their expression with various markers of T cell maturation. J Immunol 123: 2063

    PubMed  CAS  Google Scholar 

  23. Bentwich Z, Douglas SD, Siegal FP, Kunkel HG (1973) Human lymphocyte sheep erythrocyte rosette formation: some characteristics of the interaction. Clin Immunol Immunopathol 1: 511

    Article  PubMed  CAS  Google Scholar 

  24. Gallinger LA, Pross HF, Baines MG (1980) Human lymphocyte/human erythrocyte rosettes: I. Blood “H” rosettes are high affinity E-rosette-forming T lymphocytes occurring in high frequency. Int J Cancer 26: 139

    Google Scholar 

  25. Pellegrino MA, Ferrone S, Dierich MP, Reisfeld RA (1975) Enhancement of sheep red blood cell human lymphocyte rosette formation by the sulfhydril compound 2-amino-ethylisothiouronium bromide. Clin Immunol Immunopathol 3: 324

    Article  PubMed  CAS  Google Scholar 

  26. McMichael AJ, Pilch JR, Galfre G, Mason DY, Fabre JW, Milstein C (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol 9: 205

    Article  PubMed  CAS  Google Scholar 

  27. Reinherz EL, Kung PC, Goldstein G, Levy RH, Schlossman SF (1980) Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes and leukemic lymphoblasts of T cell lineage. Proc Nati Acad Sci USA 77: 1588

    Google Scholar 

  28. Richard Y, Boumsell L, Coppin H, Mishall Z, Lemerle J, Bernard A (1981) Correspondence between lectins defined and surface antigens defined cell compartments within the human thymus. Its variation during ontogeny. J Immunol 127: 252

    Google Scholar 

  29. Bhan AK, Reinherz EL, Poppema S, McCluskey RT, Schlossman SF (1980) Location of T cell and major histocompatibility antigens in the human thymus. J Exp Med 152: 771

    Article  PubMed  CAS  Google Scholar 

  30. Umiel T, Daley JF, Bhan AK, Levy RH, Schlossman SF, Reinherz EL (1982) Acquisition of immune competence by a subset of human cortical thymocytes expressing mature T cell antigens. J Immunol 129: 1054

    PubMed  CAS  Google Scholar 

  31. Gurtler LG (1981) Glycolipid and p40 are the binding sites in the sheep erythrocyte and T lymphocyte membrane responsible for rosette formation. Immunobiology 158: 426

    Article  PubMed  CAS  Google Scholar 

  32. Primi D, Lewis GK, Triglia R, Goodman JW (1979) Rosette formation between murine lymphocytes and erythrocytes. A new locus in the H2 region. J Exp Med 149: 1349

    Article  PubMed  CAS  Google Scholar 

  33. Tom onari K, Wakisaka A, Aizawa M (1980) Self recognition by autologous mixed lymphocyte reaction primed cells. J Immunol 125: 1596

    PubMed  CAS  Google Scholar 

  34. Palacios R, Llorente L, Alarcon-Segovia D, Ruiz-Arguelles A, Diaz-Jouanen E (1980) Autologous rosette-forming T cells as the responding cells in human autologous mixed lymphocyte reaction. J Clin Invest 65: 1527

    Article  PubMed  CAS  Google Scholar 

  35. Huber C, Merkenschlager M, Gattringer C, Royston I, Fink U, Braunsteiner H (1982) Human autologous mixed lymphocyte reactivity is primarily specific for xenoprotein determinants adsorbed to antigen-presenting cells during rosette formation with sheep erythrocytes. J Exp Med 155: 1222

    Article  PubMed  CAS  Google Scholar 

  36. Smolen JS, Sharrow SO, Steinberg AD (1981) Characterization of autologous rosette-forming cells: a non-restricted phenomenon. J Immunol 127: 737

    PubMed  CAS  Google Scholar 

  37. Oh SK, Moolten PC (1982) Non specific immunosuppressive factors in malignant ascites: further characterization and possible relationship to erythrocyte receptors of human peripheral T cells. J Immunol 127: 2300

    Google Scholar 

  38. Palacios R, Martinez-Maza 0 (1982) Is the E receptor on human T lymphocytes a “negative signal receptor”? J Immunol 129: 2479

    Google Scholar 

  39. Zinkernagel RM (1978) Major transplantation antigens in T cell mediated immunity: comparison of the transplantation reaction with antiviral immunity. Fed Proc 37: 2379

    PubMed  CAS  Google Scholar 

  40. (to be published) Proceedings of the First International Workshop on Human Leucocyte Differentiation Antigens (1983) Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF (eds) Springer, Berlin Heidelberg New York

    Google Scholar 

  41. Scheffel JW, Swartz SJ (1982) Inhibition of autologous rosette formation by monoclonal antibody to the sheep erythrocyte receptor. J Immunol 128: 1930

    PubMed  CAS  Google Scholar 

  42. Reinherz EL, Hussey RE, Schlossmann SF (1980) A monoclonal antibody blocking human T cell function. Eur J Immunol 10: 758

    Article  PubMed  CAS  Google Scholar 

  43. Charreire J, Bach JF (1975) Binding of autologous erythrocytes to immature T cells. Proc Nat Acad Sci USA 72: 3201

    Article  PubMed  CAS  Google Scholar 

  44. Galili U, Schlesinger M (1975) Subpopulations of human thymus cells differing in their capacity to form stable E-rosettes and their immunologic reactivity. J Immunol 115: 827

    PubMed  CAS  Google Scholar 

  45. West WH, Payne SM, Weese JL, Herberman RB (1977) Human T lymphocytes subpopulations: correlation between E rosette forming cell affinity and expression of Fc receptors. J Immunol 119: 548

    CAS  Google Scholar 

  46. Huber C, Rodt H, Thierfelder S, Braunsteiner H (1979) Surface properties and in vitro functional capacities of human T lymphocyte subsets separated by linear density gradients. J Immunol 123: 405

    PubMed  CAS  Google Scholar 

  47. Palacios R, Alarcón-Segovia D, Llorente L, Ruiz-Arguelles A, Diaz-Jouanen E (1981) Human post-thymic precursor cells in health and disease: 1. Characterization of the autologous rosette-forming T cells as post-thymic precursors. Immunology 42: 127

    Google Scholar 

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Authors
  1. M. Amiot
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  2. A. Bernard
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  3. C. Gelin
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  4. B. Raynal
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  5. L. Boumsell
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Editor information

Editors and Affiliations

  1. Institut Gustave Roussi, Service de Pédiatrie, Rue Camille Dismoulins, F-94800, Villejuif, France

    Alain Bernard

  2. INSERM U. 93, Hôpital Saint-Louis, F-75475, Paris Cedex 10, France

    Laurence Boumsell

  3. Institut de Recherches sur les Maladies du Sang, Hôpital Laint-Louis, F-75475, Paris Cedex 10, France

    Jean Dausset

  4. MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, Great Britain

    César Milstein

  5. Sidney Farber Cancer Institute, 44 Binney Street, 02115, Boston, MA, USA

    Stuart F. Schlossman

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© 1984 Springer-Verlag Berlin Heidelberg

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Amiot, M., Bernard, A., Gelin, C., Raynal, B., Boumsell, L. (1984). Phenomenon of Rosette Formation of Human T Cells Analyzed with Monoclonal Antibodies. The Same Mechanism of Interaction Accounts for Rosette Formation with Erythrocytes from Many Species, Including Autologous Erythrocytes. In: Bernard, A., Boumsell, L., Dausset, J., Milstein, C., Schlossman, S.F. (eds) Leucocyte Typing. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68857-7_15

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  • DOI: https://doi.org/10.1007/978-3-642-68857-7_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-68859-1

  • Online ISBN: 978-3-642-68857-7

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