Growth and Differentiation of Rabbit Blastocysts in Defined Culture Media

  • H. M. Beier
  • U. Mootz
  • B. Fischer
  • R. Ströbele-Müller

Abstract

The mammalian blastocyst is formed by two fundamental processes which take place in the course of embryonic cleavage. These are first, the development of a certain number of species-specific blastomeres, and second, the formation of the blastocyst’s cavity (blastocoele) following the intercellular accumulation of fluid, the origin of which is the vacuolization of the blastomeric cytoplasm and the extrusion of the content of these vacuoles. The most important step in development of the blastocyst is the differentiation of embryoblast and trophoblast cells. During cleavage, the first eight or ten blastomeres within the zona pellucida are arranged such that one or two blastomeres are inner cells and the others surround them as outer cells, forming a morula. Between the 16-and 32-cell stages the blastocyst cavity is created by coalescence of the enlarged fluid-filled intercellular spaces (Calarco and Brown 1969). When the early blastocyst stage is reached after the relatively slow process of cleavage, at the age of 3–4 days, a characteristic species-dependent growth begins. Usually, the blastocyst enters the uterus at the same time, and cellular proliferation and expansion proceed quickly up to the time of implantation. However, at the beginning of implantation the numbers of cells in blastocysts vary remarkably between species, e.g., the mouse blastocyst is comprised of about 60, that of the rabbit of more than 5000 cells.

Keywords

Estrogen Compaction Thiamine Riboflavin Pyridoxine 

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References

  1. Adams CE (1970) The development of rabbit eggs after culture in vitro for 1–4 days. J Embryol Exp Morphol 23: 21–34PubMedGoogle Scholar
  2. Beier HM (1974) Oviducal and uterine fluids. J Reprod Fertil 37: 221–237PubMedCrossRefGoogle Scholar
  3. Beier HM (1976) Uteroglobin and related biochemical changes in the reproductive tract during early pregnancy in the rabbit. J Reprod Fertil (Suppl) 25: 53–69Google Scholar
  4. Beier HM (1978) Control of implantation by interference with uteroglobin synthesis, release and utilization. In: Ludwig H, Tauber PF (eds) Human fertilization. Thieme, Stuttgart, pp 191–203Google Scholar
  5. Beier HM (1982) Uteroglobin and other endometrial proteins: Biochemistry and biological significance in beginning pregnancy. In: Beier HM, Karlson P (eds) Proteins and steroids in early pregnancy. Springer, Berlin Heidelberg New York, pp 39–71CrossRefGoogle Scholar
  6. Calarco PG, Brown EH (1969) An ultrastructural and cytological study of preimplantation development of the mouse. J Exp Zool 171: 253–284PubMedCrossRefGoogle Scholar
  7. Chang MC (1948) Transplantation of fertilized rabbit ova: The effect of viability of age, in vitro storage period, and storage temperatures. Nature 161: 978–979PubMedCrossRefGoogle Scholar
  8. Denker H-W, Gerdes H-J (1979) The dynamic structure of rabbit blastocyst coverings. I. Transformation during regular preimplantation development. Anat Embryol 157: 15–34PubMedCrossRefGoogle Scholar
  9. Ducibella T (1977) Surface changes of the developing trophoblast cell. In: Johnson MH (ed) Development in mammals, vol 1. North-Holland, Amsterdam, pp 5–30Google Scholar
  10. Ducibella T (1980) Divalent antibodies to mouse embryonal carcinoma cells inhibit compaction in the mouse embryo. Dev Biol 79: 356–366PubMedCrossRefGoogle Scholar
  11. Ducibella T, Anderson E (1975) Cell shape and membrane changes in the eight-cell mouse embryo: prerequisite for morphogenesis of the blastocyst. Dev Biol 47: 45–58PubMedCrossRefGoogle Scholar
  12. Fisher B, Denker H-W, Beier HM (1982) Incomplete transformation of rabbit embryo coverings in vitro. Annual Conf Society Study of Fertil Abstr Vol, Sutton Bonington, p 58Google Scholar
  13. Gardner RL (1971) Manipulations on the blastocyst. Adv Bio sci 6: 279–296Google Scholar
  14. Gardner RL (1972) An investigation of inner cell mass and trophoblast tissue following their isolation from the mouse blastocyst. J Embryol Exp Morphol 28: 279–312PubMedGoogle Scholar
  15. Gardner RL (1978) The relationship between cell lineage and differentiation in the early mouse embryo. In: Beermann W, Gehring WJ, Gurdon JB, Kafatos FC, Reinert J (eds) Results and problems in cell differentiation. Springer, Berlin Heidelberg New York, pp 205–241Google Scholar
  16. Kane MT, Foote RH (1970) Culture of two-and four-cell rabbit embryos to the expanding blastocyst stage in synthetic media. Proc Soc Exp Biol Med 133: 921–925PubMedGoogle Scholar
  17. Lauritsen JG (1982) The cytogenetics of spontaneous abortion. Res Reprod 14: (3) 3Google Scholar
  18. Maurer RR (1978) Advances in rabbit embryo culture. In: Daniel JC Jr (ed) Methods in mammalian reproduction. Academic, New York, pp 259–272Google Scholar
  19. Maurer RR, Beier HM (1976) Uterine proteins and development in vitro of rabbit pre-implantation embryos. J Reprod Fertil 48: 33–41PubMedCrossRefGoogle Scholar
  20. Maurer RR, Onuma H, Foote RH (1970) Viability of cultured and transferred rabbit embryos. J Reprod Fertil 21: 417–422PubMedCrossRefGoogle Scholar
  21. Naglee D, Maurer RR, Foote RH (1969) Effect of osmolarity on in vitro development of rabbit embryos in a chemically defined medium. Exp Cell Res 58: 331–333PubMedCrossRefGoogle Scholar
  22. Nicholas JS, Hall BV (1942) Experiments on developing rats. II. The development of isolated blastomeres and fused eggs. J Exp Zoo 190: 441–459CrossRefGoogle Scholar
  23. Pinsker MC, Mintz B (1973) Change in cell surface glycoproteins of mouse embryos before implantation. Proc Natl Acad Sci USA 70: 1645–1648PubMedCrossRefGoogle Scholar
  24. Rowinski J, Solter D, Koprowski H (1976) Change in concanavalin-A-induced agglutinability during preimplantation in mouse embryos. Exp Cell Res 100: 404–408PubMedCrossRefGoogle Scholar
  25. Seidel F (1952) Die Entwicklungspotenzen einer isolierten Blastomere des Zweizellenstadiums im Säugetierei. Naturwissenschaften 39: 355–356CrossRefGoogle Scholar
  26. Seidel F (1960) Die Entwicklungsfähigkeiten isolierter Furchungszellen aus dem Ei des Kaninchens (Oryctolagus cuniculus). Arch Entwicklungsmech Org 152: 43–130CrossRefGoogle Scholar
  27. Ströbele-Müller R (1983) Die Entwicklung von in-vitro-kultivierten Kaninchenblastocysten unter besonderer Berücksichtigung des FCS-Zusatzes zum Kulturmedium. Thesis Medical Faculty, Rhein-Westf Technische Hochschule, AachenGoogle Scholar
  28. Tarkowski AK (1959) Experiments on the development of isolated blastomeres of mouse egg. Nature 184: 1286–1287PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • H. M. Beier
    • 1
  • U. Mootz
    • 1
  • B. Fischer
    • 1
  • R. Ströbele-Müller
    • 1
  1. 1.Department of Anatomy and Reproductive Biology, Medical FacultyRheinisch-Westfälische Technische HochschuleAachenFederal Republic of Germany

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