Factors Controlling Normal Embryo Development and Implantation of Human Oocytes Fertilized In Vitro

  • A. Trounson

Abstract

Following the unequivocal demonstration that in vitro fertilization could be successful carried out in women given clomiphene citrate and human chorionic gonadotrophin (hCG) to induce multiple follicular growth and to control the time of oocyte recovery (Trounson et al. 1981a, b), we have attempted to determine systematically the factors which govern the success rate of in vitro fertilization. The initial experiments were aimed to improve the rate of oocyte recovery because it was considered necessary to at least begin with as many mature oocytes as possible; in particular it was hoped to have at least one oocyte for every patient undergoing laparoscopy. Changes to instruments and techniques had a marked effect on increasing the recovery rate of oocytes (Renou et al. 1981; Wood et al. 1981). It was then possible. to examine the procedures of fertilization and culture of oocytes and embryos to see if the rate of apparently normal embryo development could be increased so that the majority of patients would be transferred at least one normal embryo. Again it was demonstrated that a marked increase could be obtained in the rate of development of apparently normal embryos by the adjustment of the time of insemination (Trounson et al. 1982). With the resulting increase in the number of pregnancies obtained in the programme (Trounson 1982a) it was possible to begin detailed analyses of the factors with influence pregnancy rate, or indirectly, the rate of implantation of embryos obtained by in vitro fertilization.

Keywords

Catheter Albumin Citrate Testosterone Progesterone 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • A. Trounson
    • 1
  1. 1.Department of Obstetrics and GynaecologyMonash University, Queen Victoria and Epworth Medical CentresMelbourneAustralia

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