Differentiation of a Human Myeloid Cell Line (HL-60) Toward Granulocyte- and Macrophage-like Cells: Comparison of Cell Surface Antigen Expression
Human hematopoietic pathways have been defined mainly by the analysis of cellular morphological changes because of the paucity of other meaningful markers. Monocolonal antibodies against human cell surface antigens offer the possibility of following changes in the expression of these molecules, in particular when combined with the use of cell lines able to differentiate in vitro if provided with an appropriate stimulus. The aim of this work was to correlate antigenic changes on the surface of differentiating myelomonocytoid cells with the disappearance or appearance of morphologically distinct cell types during hematopoietic differentiation. The promyelocytic leukemia-derived cell line HL-60 was employed as an effective model system. This cell line  can be induced with retinoic acid to differentiate toward granulocytes , and after the addition of the phorbol ester TPA to HL-60 cells, they become macrophage-like . The expression of surface antigens on these cells following the different types of induction treatment was analyzed with a panel of over 70 monoclonal antibodies , using indirect immunofluorescence and bacterial binding assays.
KeywordsLeukemia Glutamine Stein Phorbol Diester
Unable to display preview. Download preview PDF.
- 5.Müller C, Ziegler A, Müller G, Schunter F, Wernet P (to be published) A monoclonal antibody (TÜ48) defining alloantigenic class I determinants specific for HLA-Bw4 and HLA-Aw-23, -A W24 as well as -Aw32. Human ImmunolGoogle Scholar
- 10.Uchańska-Ziegler B (1982) The human promyelocytic cell line HL-60 as a model for the study of granulocyte and monocyte differentiation in vitro: Selective chemical induction and phenotypic surface analysis by monoclonal antibodies. Ph.D. thesis, University of TübingenGoogle Scholar
- 14.Ziegler A, Stein H, Müller C, Wernet P (1981) TÜ1: A monoclonal antibody defining a B-cell subpopulation-usefullness for the classification of non-Hodgkin’s lymphoma. In: Knapp W (ed) Leukemia markers. Academic, London, p 113Google Scholar