Differentiation of a Human Myeloid Cell Line (HL-60) Toward Granulocyte- and Macrophage-like Cells: Comparison of Cell Surface Antigen Expression

  • B. Uchańska-Ziegler
  • P. Wernet
  • A. Ziegler
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 28)


Human hematopoietic pathways have been defined mainly by the analysis of cellular morphological changes because of the paucity of other meaningful markers. Monocolonal antibodies against human cell surface antigens offer the possibility of following changes in the expression of these molecules, in particular when combined with the use of cell lines able to differentiate in vitro if provided with an appropriate stimulus. The aim of this work was to correlate antigenic changes on the surface of differentiating myelomonocytoid cells with the disappearance or appearance of morphologically distinct cell types during hematopoietic differentiation. The promyelocytic leukemia-derived cell line HL-60 was employed as an effective model system. This cell line [4] can be induced with retinoic acid to differentiate toward granulocytes [3], and after the addition of the phorbol ester TPA to HL-60 cells, they become macrophage-like [8]. The expression of surface antigens on these cells following the different types of induction treatment was analyzed with a panel of over 70 monoclonal antibodies [12], using indirect immunofluorescence and bacterial binding assays.


Retinoic Acid Antigenic Determinant Distinct Cell Type Antigenic Change Human Myeloid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Ault KA, Springer TA (1981) Cross reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol 126:359PubMedGoogle Scholar
  2. 2.
    Barnstable CJ, Bodmer WF, Brown G, Galfrè G, Milstein C, Williams AF, Ziegler A (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens: New tools for genetic analysis. Cell 14: 9PubMedCrossRefGoogle Scholar
  3. 3.
    Breitman TR, Selonick SE, Collins SJ (1980) Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc Natl Acad Sci USA 77:2936PubMedCrossRefGoogle Scholar
  4. 4.
    Gallagher R, Collins S, Trujillo J, McCredie K, Ahearn M, Tsai S, Metzgar R, Aulakh G, Ting R, Ruscetti F, Gallo R (1979) Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54:713PubMedGoogle Scholar
  5. 5.
    Müller C, Ziegler A, Müller G, Schunter F, Wernet P (to be published) A monoclonal antibody (TÜ48) defining alloantigenic class I determinants specific for HLA-Bw4 and HLA-Aw-23, -A W24 as well as -Aw32. Human ImmunolGoogle Scholar
  6. 6.
    Perussia B, Trinchieri G, Lebman D, Jankiewicz J, Lange B, Rovera G (1982) Monoclonal antibodies that detect differentiation surface antigens of human myelomonocytic cells. Blood 59: 382PubMedGoogle Scholar
  7. 7.
    Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1979) Separation of functional subset of human T -cells by a monoclonal antibody. Proc Natl Acad Sci USA 76:4061PubMedCrossRefGoogle Scholar
  8. 8.
    Rovera G, Santoli D, Damsky C (1979) Human promyelocytic leukemia cells in culture differentiate into macrophage-like cells when treated with phorbol diester. Proc Natl Acad Sci USA 76:2779PubMedCrossRefGoogle Scholar
  9. 9.
    Stein H, Uchańska-Ziegler B, Gerdes J, Ziegler A, Wernet P (1982) Hodgkin and Sternberg-Reed cells contain antigens specific to late cells of granulopoiesis. Int J Cancer 29:283PubMedCrossRefGoogle Scholar
  10. 10.
    Uchańska-Ziegler B (1982) The human promyelocytic cell line HL-60 as a model for the study of granulocyte and monocyte differentiation in vitro: Selective chemical induction and phenotypic surface analysis by monoclonal antibodies. Ph.D. thesis, University of TübingenGoogle Scholar
  11. 11.
    Uchańska-Ziegler B, Wernet P, Ziegler A (1980) Rapid preparation of multiple cell samples for immunofluorescence analysis using microtiter plates. J Immunol Meth 39: 85CrossRefGoogle Scholar
  12. 12.
    Uchańska-Ziegler B, Wernet P, Ziegler A (1982) A single-step bacterial binding assay for the classification of cell types with surface antigen-directed monoclonal antibodies. Br J Haematol 52: 155PubMedCrossRefGoogle Scholar
  13. 13.
    Ziegler A, Milstein C (1979) A small polypeptide different from β 2-microglobulin associated with a human cell surface antigen. Nature 279:243PubMedCrossRefGoogle Scholar
  14. 14.
    Ziegler A, Stein H, Müller C, Wernet P (1981) TÜ1: A monoclonal antibody defining a B-cell subpopulation-usefullness for the classification of non-Hodgkin’s lymphoma. In: Knapp W (ed) Leukemia markers. Academic, London, p 113Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • B. Uchańska-Ziegler
  • P. Wernet
  • A. Ziegler

There are no affiliations available

Personalised recommendations