Abstract
Oncogenic transformation by tumour viruses requires, in many cases, the function of only one viral gene. In the case of the papovavirus Simian virus 40 (SV40) it is the gene coding for large T-antigen [8, 16] while for most strongly transforming retroviruses it is a gene of cellular origin [1]. Recent work has shown that many murine and human tumours, for which there is no indication of viral involvement, contain genes which have been altered in such a way that they have acquired the ability to transform the NIH3T3 line of mouse cells [3, 5, 7, 10, 15]. In order to understand the molecular mechanisms of oncogenesis it is necessary to analyse in detail the biochemical functions of the protein products of these various types of transforming gene. However, even a total description of the activities of a transforming protein will not reveal the complete mechanism of oncogenesis. Transformed cells differ from their normal parents in a multitude of biological and biochemical properties and it is unlikely that all of these changes occur as a direct result of the action of the transforming protein. Rather, the product of the oncogene must reprogramme the cell’s metabolism and/or gene expression so that having defined the transforming protein it is then necessary to identity its cellular targets.
P. M. B. and D. M. are supported by a Training Fellowship and a Research Studentship, respectively, from the Medical Research Council while K.-H. W. is supported by a Long Term Fellowship from the European Molecular Biology Organisation. P. W. J. R. holds a Career Development Award from the Cancer Research Campaign, which also paid for this work
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References
Bishop JM, Varmus H (1982) In: Weiss et al. (eds) Molecular biology of tumor viruses: RNA tumor viruses, 2nd ed. pp 999–108. Cold Spring Harbor Laboratory, New York
Clayton CE, Rigby PWJ (1981) Cell 25:547–59
Der CJ, Krontiris TG, Cooper GM (1982) Proc Natl Acad Sci USA 79: 3637–3640
Derman E, Krauter K, Walling L, Weinberger C, Ray M, Darnell JE Jr. (1981) Cell 23:731–739
Goldfarb M, Shimizu K, Perucho M, Wigler M (1982) Nature 296:404–409
Groudine M, Weintraub H (1980) Proc Natl Acad Sci USA 77:5351–5354
Lane M-A, Sainten A, Cooper GM (1982) Cell 28: 873–880
Martin RG (1981) Adv Cancer Res 34: 1–68
Noyes BE, Stark GR (1975) Cell 5:301–310
Reddy EP, Reynolds RK, Santos E, Barbacid M (1982) Nature 300: 149–152
Rigby PWJ (1979) Biochem Soc Symposium 44:89–101
Rigby PWJ, Chia W, Clayton CE, Lovett M (1980) Proc R Soc Lond Ser B 210:437–450
Rigby PWJ, Lane DP (1983) Adv Viral Oncol 3 (in press)
Soprano KJ, Jonak GJ, Galanti N, Floros J, Baserga R (1981) Virology 109: 127–136
Tabin CJ, Bradley SM, Bargmann CI, Weinberg RA, Papageorge AG, Scolnick EM, Dhar R, Lowy DR, Chang EH (1982) Nature 300: 143–149
Tooze J (1981) Molecular biology of tumor viruses. Part 2. DNA tumor viruses. Revised 2nd Edn. Cold Spring Harbor Laboratory, New York
Williams JG, Hoffman R, Penman S (1977) Cell 11: 901–907
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Scott, M.R.D., Brickell, P.M., Latchman, D.S., Murphy, D., Westphal, KH., Rigby, P.W.J. (1983). The Use of cDNA Cloning Techniques to Isolate Genes Activated in Tumour Cells. In: Neth, R., Gallo, R.C., Greaves, M.F., Moore, M.A.S., Winkler, K. (eds) Modern Trends in Human Leukemia V. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 28. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68761-7_45
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DOI: https://doi.org/10.1007/978-3-642-68761-7_45
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