Abstract
Previous attempts to immortalize cytotoxic T-lymphocytes (CTL) by somatic cell hybridization were unsuccessful (1,2), possibly due to CTL-induced nonspecific lysis of the fusion partner during the fusion process. On the other hand, the functional CTL hybridomas generated by Nabholz and colleagues (3) appeared to be T cell growth factor (TCGF) dependent, because TCGF dependent CTL lines had been used for fusion.
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References
Köhler G, Lefkovits I, Elliott B, Coutinho A (1977) Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur J Immunol 7: 758–761
Grützmann R, Hämmerling G (1978) Characterization and functional analysis of T cell hybrids. Curr Top Microbiol Immunol 81: 188–191
Nabholz M, Cianfriglia M, Acuto O, Conzelmann A, Haas W, von Boehmer H, MacDonald HR, Johnson JP (1980) Cytolytically active murine T cell hybridomas. Nature 287: 437–440
Kaufmann Y, Berke G, Eshhar Z (1981) Functional cytotoxic T lymphocyte hybridomas. Transpl Proc 13: 1171–1175
Kaufmann Y, Berke G, Eshhar Z (1981) Cytotoxic T lymphocyte hybridomas which mediate specific tumor cell lysis in vitro. Proc Natl Acad Sci USA 78: 2502–2506
Kaufmann Y, Berke G, Eshhar Z (1982) Cytolytic T cell hybridomas In: Feldmann M and Schreier M (eds) Lymphokines. Vol 5, Academic Press, New York, p 277
Nabholz M, Cianfriglia M, Acuto O, Conzelmann A, Weiss A, Haas W, von Boehmer H (1981) The production of murine cytolytic T-cell hybrids. In: Hämmerling GJ, Hämmerling U, Kearney JF (eds) Monoclonal antibodies and T-cell hybridomas. Elsevier/North-Holland, p 539
Lemke H, Hämmerling GJ, Hämmerling U (1979) Fine specificity analysis with monoclonal antibodies of antigens controlled by the major histocompatibility complex and by the Qa/TL region in mice. Immunol Rev 47: 176–206
A generous present from Dr. G. Köhler, The Basel Institute for Immunology
Ledbetter, JA, Herzenberg LA (1979) Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 362–378
Pierres M, Goridis C, Golstein P (1981) Inhibition of murine T cell mediated cytolysis and T cell proliferation by a rat monoclonal antibody immunoprecipitating two lymphoid cell surface polypeptides of 94000 and 100000 molecular weight. Eur J Immunol in press
Golstein P, Pierres M (1981) Monoclonal antibodies as probes to study the mechanism of T cell mediated cytolysis. In: Resch K, Kirchner H (eds) Mechanisms of lymphocyte activation. Elsevier/ North-Holland, p 442
Sarmiento A, Glasebrook AL, Fitch FW (1980) Monoclonal antibodies block cytolysis by T cells. J Immunol 125: 2665–2672
Hollander N, Pillemer E, Weissman I (1980) Blocking effect of Lyt 2 antibodies on T cell functions. J Exp Med 152: 674–687
Schmitt-Verhulst A-M, Sachs DH, Shearer GM (1976) Cell mediated lympholysis of TNP-modified autologous lymphocytes: Confirmation of genetic control of response to TNP-modified H-2 antigens by the use of anti-H-2 and anti-la antibodies. J Exp Med 143: 211–223
Swain SL, Dennert G, Wormsley S, Dutton RW (1981) The Lyt phenotype of a long-term allospecific T cell line. Both helper and killer activities to IA are mediated by Lyt 1 cells. Eur J Immunol 11: 175–180
Kürzinger K, Reynolds T, Germain NR, Davignon D, Martz E, Springer TA (1981) A novel lymphocyte function-associated antigen (LFA-1): Cellular distribution, quantitative expression and structure. J Immunol 127: 596–602
Golstein P, Pierres M, Schmitt-Verhulst A-M, Luciani M-F, Buferne M, Eshhar Z, Kaufmann Y (1982) Proceedings of the First International Workshop on Mechanism of Cell Mediated Cytotoxicity. Plenum Press, in press
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Eshhar, Z., Waks, T., Oren, T., Berke, G., Kaufmann, Y. (1982). Cytotoxic T Cell Hybridomas: Generation and Characterization. In: Boehmer, H.V., Haas, W., Köhler, G., Melchers, F., Zeuthen, J. (eds) T Cell Hybridomas. Current Topics in Microbiology and Immunology, vol 100. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68586-6_2
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