Analysis of the Anti-Self + TNP Immune Response: T Cell Lines, Clones and Hybridomas

  • A. M. Schmitt-Verhulst
  • M. A. Cooley
  • A. Guimezanes
  • C. De Préval
  • F. Albert
  • M. Buferne
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 100)


An immune response induced by the presentation of the hapten trinitrophenyl (TNP) on syngeneic cells can result from in vitro stimulation of T cells with syngeneic cells after trinitrobenzenesulfonate (TNBS) treatment (1) or after TNP-protein adsorption (2), or in vivo by trinitrochlorobenzene (TNCB) skin sensitization (3,4). The heterogeneity of T cell functions (5–9) is evident from the description of effector cells, including cytotoxic T cells (CTL) (1,2,4), helper T cells (TH) for CTL precursors (pCTL) (5) or B cells (6,7), as well as suppressor cells (Ts) (8–10) and delayed-type hypersensitivity (DTH) effector cells (3). In terms of the fine specificity of the response to TNBS-treated cells, it was not clear whether the apparently non-major histo-compatibility complex (MHC) -restricted components of CTL responses in mice (11,12) and humans (13) could originate from T cell recognition of non-MHC and/or nonpolymorphic cell surface antigens (13) in addition to the recognition of the known class I MHC antigens. In order to analyse such a heterogenous response in terms of functional T cell subsets (14,15) and fine specificity of T cells (16,17) we established T cells lines proliferating in response to TNBS-treated syngeneic cells (14) in which we analysed interacting T cell populations (15) and from which we derived individual T cell clones (15–17). The study of the mechanism of action of TH for the development of CTL from thymic precursor cells (5,18) led us to hope that T cell hybridomas might help in the analysis of products secreted by such TH (18). Here we will briefly summarize results obtained with such procedures.


Fine Specificity Specific Lysis Contact Sensitivity Major Histocompatibilty Complex Picryl Chloride 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • A. M. Schmitt-Verhulst
  • M. A. Cooley
  • A. Guimezanes
  • C. De Préval
  • F. Albert
  • M. Buferne

There are no affiliations available

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