Abstract
An important criterion for the use of T cell hybridomas in the study of immune responses or as a source for antigen receptors or cell recognition structures is the ability to clearly demonstrate normal immune response parameters. In studying the T cell response to cytochrome c, we have been able to show that the fine specificity of cytochrome c specific T cell hybrids resembles the response of normal T cells to this antigen. Furthermore, by holding the T cell constant we have been able to study the interaction of antigen-Ia-T cell in a way which has revealed some interesting results dealing with T cell activation and Ir gene defects.
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Solinger A.M., M.E. Ultee, E. Margoliash, R.H. Schwartz (1979) The T-lymphocyte proliferative response to pigeon cytochrome c. I.Demonstration of a T-cell heteroclitic response and identification of a single antigenic determinant whose immune recognition requires two complementing MHC-linked immune-response genes. J. Exp. Med. 150: 830
Ultee M.E., E. Margoliash, A. Lipkowski, G. Flouret, A.M. Solinger, D. Lebwohl, L.A. Matis, C. Chen, R.H. Schwartz (1980) The T lymphocyte response to cytochrome c.II.Molecular characterization of a pigeon cytochrome c determinant recognized by proliferating T lymphocytes of the B10. A mouse. Mol. Immunol. 17: 809
Hansburg D., C. Hannum, J.K. Inman, E. Appella, E. Margoliash, R.H. Schwartz (1981) Parallel cross-reactivity patterns of 2 sets of antigenically distinct cytochrome c peptides: Possible evidence for a presentational model of Ir gene function. J. Immunol. 127: 1844
Merrifield, R.B. (1969) Solid-phase peptide synthesis. Adv. Enzm. 32: 221
Heber-Katz, E., R. H. Schwartz, L. Matis, C. Hannum, T. Fairwell, E. Appella, D. Hansburg (1982) The Contribution of antigen presenting cell la to antigen-specific T cell activation. J. Exp. Med. in press
Julius, M.H., E. Simpson, L.A. Herzenberg (1973) A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur. J. Immunol. 3: 645
Rosenwasser, L.J., A.S. Rosenthal (1978) Adherent cell function in murine T lymphocyte antigen recognition.I.A macrophage dependent T cell proliferation assay in the mouse. J. Immunol. 120: 1991
Corradin, G., H.M. Etlinger, J.M. Chiller (1977) Lymphocyte specificity to protein antigens.I.Characterization of the antigen-induced in vitro T cell-dependent proliferative response with lymph node cells from primed mice. J. Immunol. 119: 1048
Click, R.E., L. Benck, B.J. Alter (1972) Immune responses in vitro.I.Culture conditions for antibody synthesis. Cell. Immunol. 3: 264
Gefter, M.C., D.H. Margulis, M.D. Scharff (1977) A simple method for poly ethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet 3: 231
Ozato, K., N. Mayer, D.H. Sachs (1980) Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and la antigens. J. Immunol. 124: 533.
Farrar, J.J., P.L. Simon, W. J. Koopman, J. Fuller-Bonar (1978) Biochemical relationship of thymocyte mitogenic factor and factors enhancing humoral and cell-mediated immune responses. J. Immunol 121: 1353
Harwell, L., B. Skidmore, P. Marrack, J. Kappler (1980) Concanavalin A- inducible,interleukin-2 producing T cell hybridoma. J. Exp. Med. 152: 893
Kappler, J.W., B. Skidmore, J. White, P. Marrack (1981) Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas: Lack of independent antigen and H-2 recognition. J. Exp. Med. 153: 1198
Longo, D.L., L.A. Matis, R.H. Schwartz (1981) Insights into immune response gene function from experiments with chimeric animals. Crit. Rev. Immunol. 2: 83
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Heber-Katz, E., Hansburg, D., Schwartz, R.H. (1982). The Effect of Antigen Presentation on the Fine Specificity of Anti-Cytochrome c T Cell Hybridomas. In: Boehmer, H.V., Haas, W., Köhler, G., Melchers, F., Zeuthen, J. (eds) T Cell Hybridomas. Current Topics in Microbiology and Immunology, vol 100. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68586-6_14
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DOI: https://doi.org/10.1007/978-3-642-68586-6_14
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