Abstract
T cells recognize antigen and self MHC products simultaneously. This phenomenon of H-2 restriction was discovered some eight years ago, but it is still not understood sufficiently (1,2). The outstanding problems appear to be the following: Do T cells have one or two receptors? Is their ligand a complex or two unconnected determinants on separate molecules? Studies with T cell hybrid clones allow one to investigate the first question by means of somatic genetics: Gene complementation between the fusion partners can reveal whether dual specificity is controlled by one or by more loci.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Zinkernagel RM, Doherty PC (1974) Restriction of in vitro T cell mediated cytotoxicity in lymphocytic choriomeningitis within a syngeneic or semiallogeneic system. Nature 248: 701–702
Shearer GM (1974) Cell mediated cytotoxicity to trinitrophenyl- modified syngeneic lymphocytes. Eur J Immunol 4: 527–533
Puri J, Lonai P (1980) Mechanism of antigen binding by T cells. H-2 (I-A) restricted binding of antigen plus la by helper cells. Eur J Immunol 10: 273–281
Lonai P, Puri J, Hämmerling GJ (1981) H-2 restricted antigen binding by a hybridoma clone which produces specific helper factor. Proc Natl Acad Sci USA 78: 549–553
Lonai P, Hämmerling GJ, Givol D, Ben-Neriah Y, Puri J (1981) Specific T-helper factor production and H-2 restricted antigen binding by helper cells: studies with T-hybridoma clones. In: Hämmerling GJ, Hämmerling H, Kearney JF (eds) Monoclonal antibodi es and T-cell hybridomas. Elsevier-North Holland, Amsterdam New York Oxford, p 457
Lonai P, Puri J, Bitton S, Ben-Neriah Y, Givol D, Hämmerling GJ (1981) H-2 restricted helper factor secreted by cloned hybridoma cells. J Exp Med 154: 942–951
Ben-Neriah Y, Wuilmart C, Lonai P, Givol D (1978) Preparation and characterization of anti framework antibodies to the heavy chain variable region (Vu) of mouse immunoglobulins. Eur J Immunol 8: 797–801
Shackelford DA, Strominger JL (1980) Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis. J Exp Med 151: 144–165
Ben-Neriah Y, Givol D, Lonai P, Simon MM, Eichmann K (1980) Allo-type linked genetic control of a polymorphic VJJ determinant on mouse T-helper cell receptors. Nature 285: 257–259
Zinkernagel RM, Callahan GN, Althage A, Cooper S, Klein PA, Klein J (1978) On the thymus in the differentiation of “H-2 self-recognition” by T cells: Evidence for dual recognition? J Exp Med 147: 882–896
v Boehmer H, Haas W, Jerne NK (1978) Major histocompatibility complex-linked immune-responsiveness is acquired by lymphocytes of low-responder mice differentiating in thymus of high-responder mice. Proc Natl Acad Sci USA 75: 2439–2442
Lonai P, Bitton S, Savelkoul HFJ, Puri J, Hammerling GJ (1981) Two separate genes regulate self-la and carrier recognition in H-2-restricted helper factors secreted by hybridoma cells. J Exp Med 154: 1910–1919
Janeway CA Jr, Wigzell H, Binz H (1976) Hypothesis: two different VIJ gene products make up the T cell receptors. Scand J Immunol 5: 993–1005
Matzinger P (1981) A one receptor view of T-cell behaviour. Nature 292: 497–501
Kappler JW, Skidmore B, White J, Marrack P (1981) Antigen inducible, h-2-restricted interleukin-2-producing T cell hybridomas. Lack of independent antigen and h-2 recognition. J Exp Med 153: 1198–1214
Pellicer A, Robins D, Wold B, Sweet R, Jackson J, Lowy I, Roberts JM, Sim GK, Silverstein S, Axel R (1980) Altering genotype and phenotype by DNA-mediated gene transfer. Science 209: 1414–1422
Harwell L, Skidmore B, Marrack P, Kappler J (1980) Concanavalin- A-inducible interleukin-2-inducing T cell hybridoma. J Exp Med 152: 893–904
Erb P, Feldmann M (1975) The role of macrophages in the generation of T helper cells. Ill Influence of macrophage-derived factors in helper cell induction. Eur J Immunol 5: 759–766
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Lonai, P., Arman, E., Bitton-Grossfeld, S., Grooten, J., Hämmerling, G. (1982). H-2-Restricted Helper Hybridomas: One Locus or Two Control Dual Specificity?. In: Boehmer, H.V., Haas, W., Köhler, G., Melchers, F., Zeuthen, J. (eds) T Cell Hybridomas. Current Topics in Microbiology and Immunology, vol 100. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68586-6_11
Download citation
DOI: https://doi.org/10.1007/978-3-642-68586-6_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-68588-0
Online ISBN: 978-3-642-68586-6
eBook Packages: Springer Book Archive