Materials and Methods
The investigations were performed on 350 Wistar rats (330 males, 20 females) and 100 NMRI mice (75 males, 25 females) of our own breeding (random-bred closed colony). The animals were housed in Makrolon cages (four rats per cage, four to six mice per cage) at 21° ± 2 °C with a 12-h light-dark cycle (light from 7 a.m. to 7 p.m., dark from 7 p.m. to 7 a.m.) and were given tap water and standard Altromin diet (TPF 1320, pelleted; mean dietary sodium 0.2%) ad libitum. The animals were selected according to age and weight (rats and mice 75-95 days old; male rats 280-300 g, female rats 190-210 g, male mice 35-45 g, female mice 30-40 g). Only estrous females were used, as determined by the cytologic examination of vaginal smears (Allen 1922).
KeywordsCryostat Section Bidistilled Water Incubation Solution Space Wire Stock Substrate Solution
Unable to display preview. Download preview PDF.