Abstract
Tissue culture techniques are today widely used in studies on the mechanism of virus replication and pathogenesis, i.e., the biochemical processes of virus multiplication in the infected cells and the different interactions between the virus components and the host organism. However, contrary to animal and bacterial virology, the plant virology section suffered for a long time from the lack of established tissue culture systems, which were suitable for biochemical or molecular biological investigations. Several attempts were made in the past to introduce plant callus cultures into plant virology (reviewed by Kassanis 1967; Hildebrandt 1977) or to use separated cells from virus-infected plants (reviewed by Zaitlin and Beachy 1974). However, all these studies contributed only a little to the understanding of functional aspects in virus multiplication. Only the recent work of H.H. Murakishi and his colleagues, who offered several promising possibilities for overcoming the limitations of cell culture systems for biochemical studies on plant viruses, revealed some new perspectives. On the other hand, plant tissue culture methods could be helpful in solving special problems such as the “hypersensitivity” of plants, which is a phenomenon primariliy related to intact tissue. Finally, plant tissue culture is of fundamental value in the production of virus-free plants through meristem tip and callus cultures (Hollings 1965), and for the mass propagation of many cultured plants.
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© 1982 Springer-Verlag Berlin Heidelberg
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Mühlbach, HP. (1982). Plant Cell Cultures and Protoplasts in Plant Virus Research. In: Cooper, M., et al. Current Topics in Microbiology and Immunology. Current Topics in Microbiology and Immunology, vol 99. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68528-6_3
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