Material and Methods

  • Walter Pfaller
Part of the Advances in Anatomy Embryology and Cell Biology book series (ADVSANAT, volume 70)


In order to obtain estimates of volume, surface and length densities of structures, at various levels of magnification, which as closely as possible resemble the kidney’s intravital architecture, several facts have to be kept in mind. Firstly the kidney must be denaturated by chemical fixatives as quickly as possible to prevent cellular damage. Secondly the fixative administered must not induce volume changes in any one of the four basic tissue components outlined in the preceding chapter. This means that the fixative composition has to be carefully adjusted to conditions which correspond to that of the cell’s natural environment. Thirdly the quantitative interrelationship between tissue components is highly dependent on the blood pressure and filtration performance. Therefore physiologic conditions must be maintained until denaturation by the fixative starts.


Glomerular Filtration Rate Maleic Acid Kidney Cortex Sampling Level Outer Medulla 
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Copyright information

© Springer Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • Walter Pfaller
    • 1
  1. 1.Institut für Physiologie und BalneologieUniversität InnsbruckInnsbruckÖsterreich

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