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Algal Walls — Cytology of Formation

  • D. G. Robinson
Part of the Encyclopedia of Plant Physiology book series (PLANT, volume 13 / B)

Abstract

Although the production of nonfibrillar extracellular polysaccharides is a feature of nearly all algal cells and indeed attains industrial importance in some instances (kelps), comparatively few cytological studies have been carried out with respect to their sites of synthesis and modes of liberation. Nevertheless it is clear that elements of the endomembrane system (endoplasmic reticulum, ER; Golgi apparatus, GA) are principally involved. Autoradiography (Evans et al. 1974) and comparative micromorphometry of secretory and nonsecretory stages (Ramus 1972, Ramus and Robins 1975) have implicated the GA in red algal mucilage production. The participation of ER and GA in the production of the glycoprotein adhesive secreted by the ship-fouling green alga Enteromorpha has been demonstrated by cytochemical (for the carbohydrate moiety) and autoradiographic (for the protein moiety) means (Callow and Evans 1977). Ultrastructural, autoradiographical, and microanalytical (for sulfur detection in sulfated polysaccharides) evidence pertaining to GA participation in slime-polysaccharide secretion has also been provided for various representatives of the brown algae (Evans and Holligan 1972, Evans et al. 1973, Evans and Callow 1974, Callow and Evans 1973, 1976). Analysis of isolated dictyosomal fractions from Fucus zygotes (Callow et al. 1978, Coughlan and Evans 1978) has furthermore shown this fraction to be the first labeled when 35S-sulfate is applied exogenously. Moreover this fraction contains an enzyme responsible for the transfer of galactose from UDP-galactose to fucose and fucoidan.

Keywords

Cortical Microtubule Centric Diatom Terminal Complex Secondary Wall Formation Microfibril Orientation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Allen DM, Northcote DH (1975) The scales of Chrysochromulina chiton. Protoplasma 83: 389–412CrossRefGoogle Scholar
  2. Atkinson AW, Gunning BES, John PCL, McCullough W (1971) Centrioles and microtubules in Chlorella. Nature (London) New Biol 234: 24–25Google Scholar
  3. Atkinson AW, Gunning BES, John PCL (1972) Sporopollenin in the cell wall of Chlorella and other algae: Ultrastructure, chemistry and incorporation of 14C-acetate, studied in synchronous cultures. Planta 107: 1–32CrossRefGoogle Scholar
  4. Barnett JR, Preston RD (1970) Arrays of granules associated with the plasmalemma in swarmers of Cladophora. Ann Bot 34: 1011–1017Google Scholar
  5. Branton D, Bullivant S, Gilula NB, Karnovsky MJ, Moor H, Mühlethaler K, Northcote DH, Packer L, Satir B, Satir P, Speth V, Staehelin LA, Weinstein RS (1975) Freeze-etching nomenclature. Science 190: 54–56PubMedCrossRefGoogle Scholar
  6. Brown RM (1969) Observations on the relationship of the Golgi apparatus to wall formation in the marine chrysophycean alga Pleurochrysis scherffelii Pringsheim. J Cell Biol 41: 109–123PubMedCrossRefGoogle Scholar
  7. Brown RM, Montezinos D (1976) Cellulose microfibrils: visualization of biosynthetic and orienting complexes in association with the plasma membrane. Proc Natl Acad Sci USA 73: 143–147PubMedCrossRefGoogle Scholar
  8. Brown RM, Romanovicz DK (1976) Biogenesis and structure of Golgi-derived cellulosic scales in Pleurochrysis. I. Role of the endomembrane system in scale assembly and exocytosis. Appl Polym Symp 28: 537–585Google Scholar
  9. Brown RM, Franke WW, Kleinig H, Falk H, Sitte P (1970) Scale formation in chrysophycean algae. I. Cellulosic and non-cellulosic wall components made by the Golgi apparatus. J Cell Biol 45: 246–271PubMedCrossRefGoogle Scholar
  10. Brown RM, Herth W, Franke WW, Romanovicz D (1973) The role of the Golgi apparatus in the biosynthesis and secretion of a cellulosic glycoprotein in Pleurochrysis: A model system for the synthesis of structural polysaccharides. In: Loewus F (ed) Biogenesis of Plant Cell Wall Polysaccharides. Academic Press, New York, pp 207–257Google Scholar
  11. Callow ME, Evans LV (1973) Studies on the ship-fouling alga Enteromorpha. III. Cytochemistry and autoradiography of adhesive production. Protoplasma 80: 15–27CrossRefGoogle Scholar
  12. Callow ME, Evans LV (1976) Localization of sulfated polysaccharides by X-Ray microanalysis in Laminaria saccharina. Planta 131: 155–157CrossRefGoogle Scholar
  13. Callow ME, Evans LV (1977) Studies on the ship-fouling alga Enteromorpha (Chlorophyceae, Ulvales). IV. Polysaccharide and nucleoside diphosphatase localization. Protoplasma 16: 313–320Google Scholar
  14. Callow ME, Coughlan SJ, Evans LV (1978) The role of Golgi bodies in polysaccharide sulfation in Fucus zygotes. J Cell Sci 32: 337–356PubMedGoogle Scholar
  15. Catt JW, Hills GJ, Roberts K (1976) A structural glycoprotein, containing hydroxyproline, isolated from the cell wall of Chlamydomonas reinhardii. Planta 131: 165–171CrossRefGoogle Scholar
  16. Catt JW, Hills GJ, Roberts K (1978) Cell wall glycoproteins from Chlamydomonas reinhardii, and their self assembly. Planta 138: 91–98CrossRefGoogle Scholar
  17. Coughlan S, Evans LV (1978) Isolation and characterization of Golgi bodies from vegetative tissue of the brown alga Fucus serratus. J Exp Bot 29: 55–68CrossRefGoogle Scholar
  18. Darley WM, Porter D, Fuller MS (1969) Cell wall composition and synthesis via Golgi-directed scale formation in the marine eucaryote, Schizochytrium aggregatum, with a note on Thraustochytrium sp. Arch Mikrobiol 90: 89–106CrossRefGoogle Scholar
  19. Darley WM, Porter D, Fuller MS (1969) Cell wall composition and synthesis via Golgi-directed scale formation in the marine eucaryote, Schizochytrium aggregatum, with a note on Thraustochytrium sp. Arch Mikrobiol 90: 89–106CrossRefGoogle Scholar
  20. Davies DR (1972) Electrophoretic analyses of wall glycoproteins in normal and mutant cells. Exp Cell Res 73: 512–516PubMedCrossRefGoogle Scholar
  21. Dobberstein B, Kiermayer O (1972) Das Auftreten eines besonderen Typs von Golgivesikeln während der Sekundärwandbildung vom Micrasterias denticulata Bréb. Protoplasma 75: 185–194CrossRefGoogle Scholar
  22. Dustin P (1978) Microtubules. Springer, Berlin Heidelberg New YorkGoogle Scholar
  23. Evans LV, Callow ME (1974) Polysaccharide sulphation in Laminaria. Planta 117: 93–95CrossRefGoogle Scholar
  24. Evans LV, Holligan MS (1972) Correlated light and electron microscope studies on brown algae. I. Localization of alginic acid and sulphated polysaccharides in Dictyota. New Phytol 71: 1161–1172CrossRefGoogle Scholar
  25. Evans LV, Simpson M, Callow ME (1973) Sulphated polysaccharide synthesis in brown algae. Planta 110: 237–252CrossRefGoogle Scholar
  26. Evans LV, Callow ME, Percival E, Fareed V (1974) Studies on the synthesis and composition of extracellular mucilage in the unicellular red alga Rhodella. J Cell Sci 16: 1–21PubMedGoogle Scholar
  27. Franke WW, Brown RM (1971) Scale formation in chrysophycean algae. III. Negatively stained scales of the coccolithophorid Hymenomonas. Arch Mikrobiol 77: 12–19CrossRefGoogle Scholar
  28. Furtado JS, Olive LS (1971) Ultrastructure of the protostelid Ceratiomyxella tahitiensis including scale formation. Nova Hedwigia 21: 537–576Google Scholar
  29. Giddings TH, Brower DL, Staehelin LA (1980) Visualization of particle complexes in the plasma membrane of Micrasterias denticulata associated with the formation of cellulose fibrils in primary and secondary walls. J Cell Biol 84: 327–339PubMedCrossRefGoogle Scholar
  30. Gooday GW (1971) A biochemical and autoradiographic study of the role of the Golgi bodies in thecal formation in Platymonas tetrahele. J Exp Bot 22: 959–971CrossRefGoogle Scholar
  31. Grimm I, Sachs H, Robinson DG (1976) Structure, synthesis and orientation of microfibrils. II. The effect of colchicine on the wall of Oocystis solitaria. Cytobiologie 14: 61–74Google Scholar
  32. Heath IB (1974) A unified hypothesis for the role of membrane bound enzyme complexes and microtubules in plant cell wall synthesis. J Theoret Biol 48: 445–449CrossRefGoogle Scholar
  33. Hertel C, Quader H, Robinson DG, Marmé D (1980) Antimicrotubule herbicides and fungicides affect Ca2+ — transport in plant mitochondria. Planta 149: 336–340CrossRefGoogle Scholar
  34. Herth W (1979) The site of β-chitin fibril formation in centric diatoms. II. The chitin-forming cytoplasmic structures. J Ultr Res 68: 16–27CrossRefGoogle Scholar
  35. Herth W, Barthlott W (1979) The site of β-chitin fibril formation in centric diatoms. I. Pores and fibril formation. J Ultr Res 68: 6–15CrossRefGoogle Scholar
  36. Herth W, Zugenmaier P (1977) Ultrastructure of the chitin microfibrils of the centric diatom Cyclotella cryptica. J Ultr Res 61: 230–239CrossRefGoogle Scholar
  37. Herth W, Franke WW, Stadler J, Bittiger H, Keilich G, Brown RM (1972) Further characterization of the alkali-stable material from the scales of Pleurochrysis scherfellii: a cellulosic glycoprotein. Planta 105: 72–92CrossRefGoogle Scholar
  38. Herth W, Kuppel A, Schnepf E (1977) Chitinous fibrils in the lorica of the flagellate chrysophyte Poterioochromonas stipitata (syn. Ochromonas malhamensis). J Cell Biol 73: 311–321PubMedCrossRefGoogle Scholar
  39. Hibberd DJ (1976) The ultrastructure and taxonomy of the Chrysophyceae and Prymnesiophyceae (Haptophyceae) : a survey with some new observations on the ultrastructure of the Chrysophyceae. Bot J Linn Soc 72: 55–80CrossRefGoogle Scholar
  40. Hills GJ, Philips JM, Gay MR, Roberts K (1975) Self-assembly of a plant cell wall in vitro. J Mol Biol 96: 431–441PubMedCrossRefGoogle Scholar
  41. Kiermayer O (1968) The distribution of microtubules in differentiating cells of Micrasterias denticulata Bréb. Planta 83: 223–236CrossRefGoogle Scholar
  42. Kiermayer O (1977) Biomembran als Träger morphogenetischer Information. Untersuchungen bei der Grünalge Microasterias. Naturwiss Rundsch 30: 161–165Google Scholar
  43. Kiermayer O, Dobberstein B (1973) Membrankomplex dictyosomaler Herkunft als „Matritzen“ für die extraplasmatische Synthese und Orientierung von Mikrofibrillen. Protoplasma 77: 437–451CrossRefGoogle Scholar
  44. Kiermayer O, Sleytr UB (1979) Hexagonally ordered “rosettes” of particles in the plasma membrane of Micrasterias denticulata Bréb. and their significance for microfibril formation and orientation. Protoplasma 101: 133–138CrossRefGoogle Scholar
  45. Leadbeater BSC (1971) Observations on the life history of the haptophycean alga Pleurochrysis scherffelii with special references to the microanatomy of the different types of motile cells. Ann Bot 35: 429–439Google Scholar
  46. MacDonald K, Pickett-Heaps JD (1976) Ultrastructure and differentiation in Cladophora glomerata. I. Cell division. Am J Bot 63: 592–601CrossRefGoogle Scholar
  47. MacLachlan J, Mclnnes AG, Falk M (1965) Studies on the chitin (chitin-poly-N-acetyl-glucosamine) fibers of the diatom Thalasiosira fluviatilis Hustedt. I. Production and isolation of chitin fibers. Can J Bot 43: 707–713CrossRefGoogle Scholar
  48. Manton I (1966) Observations on scale production in Pyramimonas amylifera Conrad. J Cell Sci 1: 429–438PubMedGoogle Scholar
  49. Manton I (1967) Further observations on the fine structure of Chrysochromulina chiton with special reference to the haptonema, “peculiar” Golgi structure and scale production. J Cell Sci 2: 265–272PubMedGoogle Scholar
  50. Manton I (1972) Observations on the biology and micro-anatomy of Chrysochromulina megacylindrica. Br Phycol J 7: 235–248CrossRefGoogle Scholar
  51. Marchant HJ, Pickett-Heaps JD (1972) Ultrastructure and differentiation of Hydrodictyon reticulatum. III. Formation of the vegetative daughter net. Aust J Biol Sci 25: 265–278Google Scholar
  52. Minami SA, Goodenough UW (1978) Novel glycopolypeptide synthesis induced by gametic cell fusion in Chlamydomonas reinhardii. J Cell Biol 77: 165–181PubMedCrossRefGoogle Scholar
  53. Mix M (1972) Die Feinstruktur der Zellwände bei Mesotaeniceae und Gonatozygaceae mit einer vergleichenden Betrachtung der verschiedenen Wandtypen der Conjugatophyceae und deren systematischen Wert. Arch Mikrobiol 81: 197–220CrossRefGoogle Scholar
  54. Mix M, Manshard E (1977) Über Mikrofibrillen-Aggregate in langgestreckten Vesikeln und ihre Bedeutung für die Zellwandbildung bei einem Stamm von Penium (Desmidiales). Ber Dtsch Bot Ges 90: 517–526Google Scholar
  55. Moestrup O, Thomsen HA (1974) An ultrastructural study of the flagellate Pyramimonas orientalis with particular emphasis on Golgi apparatus activity and the flagellar apparatus. Protoplasma 81: 247–269CrossRefGoogle Scholar
  56. Moestrup O, Walne PL (1979) Studies on scale morphogenesis in the Golgi apparatus of Pyraminonas tetrahynchus (Prasinophyceae). J Cell Sci 36:437–459PubMedGoogle Scholar
  57. Montezinos D, Brown RM (1976) Surface architecture of the plant cell: biogenesis of the cell wall, with special emphasis on the role of the plasma membrane in cellulose biosynthesis. J Supramol Struct 5: 277–290PubMedCrossRefGoogle Scholar
  58. Montezinos D, Brown RM (1978) Cell wall biogenesis in Oocystis: experimental alteration of microfibril assembly and orientation. Cytobios 23: 119–139PubMedGoogle Scholar
  59. Nicolai E, Preston RD (1959) Cell wall studies in the Chlorophyceae. III. Differences in structure and development in the Cladophoraceae. Proc Roy Soc London Ser B 151: 244–255CrossRefGoogle Scholar
  60. Outka DE, Williams DC (1971) Sequential coccolith morphogenesis in Hymenomonas carterae. J Protozool 18: 285–297PubMedGoogle Scholar
  61. Peng HB, Jaffe LF (1976) Cell wall formation in Pelvetia embryos. A freeze-fracture study. Planta 133: 57–71CrossRefGoogle Scholar
  62. Pickett-Heaps JD, Staehelin LA (1975) The ultrastructure of Scenedesmus (Chlorophyceae). II. Cell division and colony formation J Phycol 11: 186–202Google Scholar
  63. Preston RD (1964) Structural and mechanical aspects of plant cell walls with particular reference to synthesis and growth. In: Zimmermann MH (ed) The formation of wood in forest trees. Academic Press, New York, pp 169–188Google Scholar
  64. Preston RD (1974) The Physical Biology of Plant Cell Walls. Chapman and Hall, LondonGoogle Scholar
  65. Quader H (1981)Effects of D2O on microtubules and microfibrils in Oocystis. In: Robinson DG, Quader H (eds) Cell walls 81. Wiss Verlagsges, Stuttgart, pp. 198–205Google Scholar
  66. Quader H, Robinson DG (1979) Structure, synthesis and orientation of microfibrils. VI. The role of ions in microfibril deposition in Oocystis solitaria. Eur J Cell Biol 20: 51–56PubMedGoogle Scholar
  67. Quader H, Wagenbreth I, Robinson DG (1978) Structure, synthesis and orientation of microfibrils. V. On the recovery of Oocystis solitaria from microtubule inhibitor treatment. Cytobiologie 18: 39–51PubMedGoogle Scholar
  68. Ramus J (1972) The production of extracellular polysaccharide by the unicellular red alga Porphyridium aerugineum. J Phycol 8: 97–111Google Scholar
  69. Ramus J, Robins DM (1975) The correlation of Golgi activity and polysaccharide secretion in Porphyridium. J Phycol 11: 70–74Google Scholar
  70. Roberts K (1974) Crystalline glycoprotein cell walls of algae: their structure, composition and assembly. Philos Trans R Soc London Sev B 268: 129–146CrossRefGoogle Scholar
  71. Roberts K, Gurney-Smith M, Hills GS (1972) Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardii. I. Ultrastructure and preliminary chemical analysis. J Ultr Res 40: 599–613CrossRefGoogle Scholar
  72. Robinson DG (1977) Structure, synthesis and orientation of microfibrils. IV. Microtubules and microfibrils in Glaucocystis. Cytobiologie 15: 475–484Google Scholar
  73. Robinson DG, Herzog W (1977) Structure, synthesis and orientation of microfibrils. III. A survey of the action of microtubule inhibitors on microtubules and microfibril orientation in Oocystis solitaria. Cytobiologie 15: 463–474Google Scholar
  74. Robinson DG, Preston RD (1971) Fine structure of swarmers of Cladophora and Chaetomorpha. I. The plasmalemma and Golgi apparatus in naked swarmers. J Cell Sci 9: 581–601PubMedGoogle Scholar
  75. Robinson DG, Preston RD (1972) Plasmalemma structure in relation to microfibril biosynthesis in Oocystis. Planta 104: 234–246CrossRefGoogle Scholar
  76. Robinson DG, Quader H (1980) Structure, synthesis and orientation of microfibrils. VII. Microtubule reassembly in vivo after cold treatment in Oocystis and its relevance to microfibril orientation. Eur J Cell Biol 21: 229–230PubMedGoogle Scholar
  77. Robinson DG, Schlösser UG (1978) Cell wall regeneration by protoplasts of Chlamydomonas. Planta 141: 83–92CrossRefGoogle Scholar
  78. Robinson DG, White RK, Preston RD (1972) Fine structure of swarmers of Cladophora and Chaetomorpha. III. Wall synthesis and development. Planta 107: 131–144CrossRefGoogle Scholar
  79. Robinson DG, Grimm I, Sachs H (1976) Colchicine and microfibril orientation. Protoplasma 89: 375–380CrossRefGoogle Scholar
  80. Romanovicz DK, Brown RM (1976) Biogenesis and structure of Golgi-derived cellulosic scales in Pleurochrysis. II. Scale composition and supramolecular structure. Appl Polym Symp 28: 587–610Google Scholar
  81. Sachs H, Grimm I, Robinson DG (1976) Structure synthesis and orientation of microfibrils. I. Architecture and development of the wall of Oocystis solitaria. Cytobiologie 14: 49–60Google Scholar
  82. Sassen A, Eyden-Emons van A, Lamers A, Wanka F (1970) Cell wall formation in Chlorella pyrenoidosa: a freeze etching study. Cytobiologie 1: 373–382Google Scholar
  83. Schnepf E, Deichgräber G (1969) Uber die Feinstrukturen von Synura petersenii unter besonderer Berücksichtigung der Morphogenese ihrer Kieselschuppen. Protoplasma 68: 85–106CrossRefGoogle Scholar
  84. Schnepf E, Röderer G, Herth W (1975) The formation of the fibrils in the lorica of Poterioochromonas stipitata: tip growth, kinetics, site, orientation. Planta 125: 45–62CrossRefGoogle Scholar
  85. Schnepf E, Deichgräber G, Röderer G, Herth W (1977) The flagellar root apparatus, the microtubular system and associated organelles in the chrysophycean flagellate Poterioochromonas malhamensis Peterfi (syn Poterioochromonas stipitata Scherffel and Ochromonas malhamensis Pringsheim). Protoplasma 92: 87–107CrossRefGoogle Scholar
  86. Staehelin LA (1966) Die Ultrastruktur der Zellwand und des Chloroplasten von Chlorella. Z Zellforsch 74: 325–350PubMedCrossRefGoogle Scholar
  87. Staehelin LA, Pickett-Heaps JD (1975) The ultrastructure of Scenedesmus (Chlorophyceae). I. Species with the “Reticulate” or “warty” type of ornamental layer. J Phycol 11: 163–185Google Scholar

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© Springer-Verlag Berlin · Heidelberg 1981

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  • D. G. Robinson

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