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Radioimmunologic Methods

  • Chapter

Part of the Handbook of Experimental Pharmacology book series (HEP,volume 56 / 1)

Abstract

Based on the work of Yalow and Berson (1959) on the detection of insulin, radioimmunoassay (RIA) has now developed to be the best and most versatile method for the detection of trace amounts of biologically active substances (hereinafter termed antigens). To enable the detection of an antigen using RIA, the following conditions must be fulfilled

  1. a)

    Availability of purified antigen for standardization

  2. b)

    Availability of antibodies reacting specifically with the antigen to be determined

  3. c)

    Availability of radioactively labeled antigen with sufficient specific activity

  4. d)

    Availability of a system allowing separation of the antibody-bound and free antigen.

Keywords

  • Cardiac Glycoside
  • Plasma Digoxin
  • Sodium Metabisulphite
  • Tritiated Digoxin
  • Tyrosine Methyl Ester

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Stellner, K. (1981). Radioimmunologic Methods. In: Greeff, K. (eds) Cardiac Glycosides. Handbook of Experimental Pharmacology, vol 56 / 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68163-9_5

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