Advertisement

Purification by Affinity Chromatography of Red Cell Membrane Acetylcholinesterase

  • U. Brodbeck
  • R. Gentinetta
  • P. Ott

Abstract

Over the past the methods for purifying integral membrane proteins have advanced considerably. In particular the onset of affinity chromatography (Jakoby and Wilchek 1974) has brought new incentives to this particular class of proteins. In order to be deployed successfully the following criteria have to be met: (A) A high affinity ligand covalently linked to an insoluble support via a spacer arm must warrant biospecific adsorption. After removal of all undesired protein, desorption should be carried out using similar biospecificity. (B) The amphiphilic membrane protein must be kept in solution throughout the purification procedure by the use of a suitable detergent. Alternatively membrane proteins may be solubilized by proteolytic enzymes, chaotropic ions or extraction with organic solvents.

Keywords

Acetic Anhydride Critical Micellar Concentration Succinic Anhydride Cyanogen Bromide Affinity Resin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Berman JD, Young M (1971) Proc Natl Acad Sci USA 68:395–398PubMedCrossRefGoogle Scholar
  2. Ellmann GL, Courtney DK, Andres V, Featherstone RM (1961) Biochem Pharmacol 7: 88–95CrossRefGoogle Scholar
  3. Frenkel EJ, Roelofsen B, Brodbeck U, van Deenen LLM, Ott P (1980) Eur J Biochem 109:377–382PubMedCrossRefGoogle Scholar
  4. Hall ER, Brodbeck U (1978) Eur J Biochem 89:159–167PubMedCrossRefGoogle Scholar
  5. Jakoby WB, Wilchek M (eds) (1974) Methods in enzymology, vol 34. Academic Press, New YorkGoogle Scholar
  6. Ott P, Brodbeck U (1978) Eur J Biochem 88:119–125PubMedCrossRefGoogle Scholar
  7. Ott P, Jenny B, Brodbeck U (1975) Eur J Biochem 57:469–480PubMedCrossRefGoogle Scholar
  8. Römer-Lüthi CR, Hajdu J, Brodbeck U (1979) Hoppe-Seyler’s Z Physiol Chem 360: 929–934PubMedCrossRefGoogle Scholar
  9. Römer-Lüthi CR, Ott P, Brodbeck U (1980) Biochim Biophys Acta 601:123–133PubMedCrossRefGoogle Scholar
  10. Wiedmer T, Di Francesco C, Brodbeck U (1979) Eur J Biochem 102:59–64PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • U. Brodbeck
  • R. Gentinetta
  • P. Ott

There are no affiliations available

Personalised recommendations