6-Benzylaminopurine as a Growth Factor for Drosophila melanogaster Cells Grown in Vitro

  • J. L. Becker
  • J. Roussaux
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Cytokinins are powerful activators of the plant growth and morphogenesis (Skoog and Armstrong, 1970; Hall, 1973). Most of them are adenine derivatives with a substitution on the 6th position of the purinic nucleus (Skoog and Armstrong, 1970). Numerous experiments have reported the biological effects of cytokinins, especially on cellular division, and described some biochemical modifications induced by such molecules. However, their precise action remains unknown. An essential property of the cytokinins will be considered: some of them are potent biosynthetic activators and, for instance, allow the growth in vitro of auxin or thiamin-dependent tissues on medium free of these components (Digby and Skoog, 1966; Stoutemyer and Britt, 1969; Jordan and Skoog, 1971; Syono and Furuya, 1972). 6-substituted adenines, as bases or nucleosides, also interfere in the division of animal cells (Buckley et al., 1962; Grillo and Polsky, 1966; Gallo et al., 1969; Rebhun and Monroy, 1970; Dorée and Guerrier, 1975), often by inhibiting it. Experiments on animal cells were mostly performed in vitro on malignant cell lines or on the whole animals using high cytokinin concentrations. In order to look for activator effects on animals similar to those described on plants, we have tested the action of small cytokinin concentrations on nonmalignant cells with important nutritional requirements. This paper demonstrates that 6-benzylaminopurine (6-BAP) is a growth factor for Drosophila melanogaster ceËs grown in vitro, and describes some effects of the cytokinin.


Drosophila Cell Nucleotidase Activity Tobacco Callus Malignant Cell Line Phosphoribosyl Pyrophosphate 
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Copyright information

© Springer-Verlag, Berlin Heidelberg 1981

Authors and Affiliations

  • J. L. Becker
    • 1
  • J. Roussaux
    • 2
  1. 1.Laboratoire de Zoologie — ERA 615Université Pierre et Marie CurieParisFrance
  2. 2.Laboratoire de Biologie Végétale IVUniversité Pierre et Marie CurieParisFrance

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