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Photoaffinity Labeling Reagents to Detect Binding of Cytokinins: Chemical Limitations

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Metabolism and Molecular Activities of Cytokinins

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

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Abstract

Photoaffinity labeling has been a powerful technique for studying ligand-receptor interactions. The versatility of this technique has been illustrated in several reviews (Chowdhry and Westheimer, 1979); (Bayley and Knowles, 1977); (Katzenellenbogen, 1976); (Cooperman, 1975); (Creed, 1974); (Knowles, 1972).

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References

  • Bayley H, Knowles JR (1977) Photoaffinity labeling. Methods Enzymol 46: 69–114

    Article  PubMed  CAS  Google Scholar 

  • Chowdhry V, Westheimer FG (1979) Photoaffinity labeling of biological systems. Annu Rev Biochem 48: 293–325

    Article  PubMed  CAS  Google Scholar 

  • Cooperman BS (1975) Photoaffinity labeling of proteins and more complex receptors. In: Smith KC (ed) Aging, carcinogenesis and radiation biology. Plenum Press Corp, New York, p 315

    Google Scholar 

  • Creed D (1974) Photochemical probes for biological interactions. Photochem Photobiol 19: 459–462

    Article  PubMed  CAS  Google Scholar 

  • Doering WE von, Odum RA (1966) Ring enlargement in the photolysis of phenyl azide. Tetrahedron 22: 81–93

    Article  CAS  Google Scholar 

  • Dreyfuss G, Schwartz K, Blout ER, Barrio JR, Liu FT, Leonard NJ (1978) Fluorescent photoaffinity labeling: Adenosine 3’,5’-cyclic monophosphate receptor sites. Proc Natl Acad Sci USA 75: 1199–1203

    Article  PubMed  CAS  Google Scholar 

  • Fox JE, Erion JL, Mc Chesney JD (1979) A new intermediate in the synthesis of a tritiated cytokinin with high specific actiivity. Phytochemistry 18: 1055–1056

    Article  CAS  Google Scholar 

  • Katzenellenbogen J A (1976) Affinity labeling as a technique in determining hormone mechanisms. In: Litwack G (ed) Biochemical action of hormones, vol IV. Academic Press, London, New York, p 1

    Google Scholar 

  • Knowles JR (1972) Photogenerated reagents for biological receptor-site labeling. Acc Chem Res 5: 155–160

    Article  CAS  Google Scholar 

  • Lwowski W (1970) Nitrenes. Wiley-Interscience, New York, pp 185–224

    Google Scholar 

  • Mc Robbie IM, Meth-Cohn O, Suschitzky H (1976) Competitive cyclizations of singlet and triplet nitrenes Part 1. Cyclization of l-(2-nitrenophenyl) pyrazoles. Tetrahadron Lett 925–928; part 2. Cyclization of 2-nitrenophenyl-thiophens, -benzothiazoles and -benzimidazoles. Tetrahedron Lett 929–932

    Google Scholar 

  • Moore FH (1979) A cytokinin-binding protein from Wheat-Germ. Isolation by affinity chromatography and properties. Plant Physiol 64: 595–599

    Google Scholar 

  • Mornet R, Theiler JB, Leonard NJ, Schmitz RY, Moore FH, Skoog F (1979) Active cytokinins. Photoaffinity labeling agents to detect binding. Plant Physiol 64: 600–610

    Article  PubMed  CAS  Google Scholar 

  • Reiser A, Wagner HM (1971) Photochemistry of the azido group. In: Patai S (ed) The chemistry of the azido group. Wiley-Interscience, New York pp 441–501

    Chapter  Google Scholar 

  • Sprecker MA, Morrice AG, Gruber BA, Leonard NJ, Schmitz RY, Skoog F (1976) Fluorescent cytokinins: stretched-out analogs of N6-benzyladenine and N6-(Δ2-isopentenyl)adenine. Phytochemistry 15: 609–613

    Article  CAS  Google Scholar 

  • Sundberg RJ, Smith RH (1971) Nucleophilic aromatic substitution during deoxygenation. Deoxygenation of nitrosobenzene by triethyl phosphite in alcohols. J Org Chem 36: 295–300

    Article  CAS  Google Scholar 

  • Sussman MR, Firn R (1976) The synthesis of radioactive cytokinin with high specific activity. Phytochemistry 15: 153–155

    Article  CAS  Google Scholar 

  • Sussman MR, Kende H (1977) The synthesis and biological properties of 8-azido-N6-benzyladenine, a potential photoaffinity reagent for cytokinin. Planta 137: 91–96

    Article  CAS  Google Scholar 

  • Temple C, Thorpe MC, Coburn WC, Montgomery JA (1966) Studies on the azidoazomethine-tetrazole equilibrium. IV-Azidopurines. J Org Chem 31: 935–938.

    Article  CAS  Google Scholar 

  • Temple C, Kussner CL, Montgomery JA (1966) V-2- and 6-azidopurines. J Org Chem 31: 2210–2215

    Article  CAS  Google Scholar 

  • Theiler JB (1977) Synthesis of purines derivatives as probes of hormones receptors. Ph D Thesis, Univ Illionis-Urbana-Champaign

    Google Scholar 

  • Theiler JB, Leonard NJ, Schmitz RY, Skoog F (1976) Photoaffinity-labeled cytokinins. Synthesis and biological activity. Plant Physiol 58: 803–805

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratoire de Chimie Organique, I.R.S.T., Université d’Angers, 49045, Angers Cedex, France

    R. Mornet

  2. Roger Adams Laboratory, School of Chemical Sciences, University of Illinois, Urbana, IL, 61801, USA

    J. B. Theiler & N. J. Leonard

Authors
  1. R. Mornet
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  2. J. B. Theiler
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  3. N. J. Leonard
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Editor information

Editors and Affiliations

  1. Laboratoire de Génétique et Physiologie du Développement des Plantes, CNRS, F-91190, Gif-sur-Yvette, France

    Jean Guern

  2. Faculté des Sciences de Luminy, Case 901 Laboratoire de Biochimie, Fonctionnelle des Plantes, ER 104 CNRS, F-13288, Marseille Cedex 9, France

    Claude Péaud-Lenoël

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© 1981 Springer-Verlag, Berlin Heidelberg

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Mornet, R., Theiler, J.B., Leonard, N.J. (1981). Photoaffinity Labeling Reagents to Detect Binding of Cytokinins: Chemical Limitations. In: Guern, J., Péaud-Lenoël, C. (eds) Metabolism and Molecular Activities of Cytokinins. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68035-9_14

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  • DOI: https://doi.org/10.1007/978-3-642-68035-9_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-68037-3

  • Online ISBN: 978-3-642-68035-9

  • eBook Packages: Springer Book Archive

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