General Methods in Scanning Electron Microscopy of the Nervous System

  • K. Meller


The scanning electron microscope (SEM) allows one to obtain a three-dimensional representation of biological structures. In this way the SEM study of nervous structures may have filled the gap between the information provided by the classic Golgitechnique and the fine structural data supplied by transmission electron microscopy. One drawback of the SEM is that it provides most information about free surfaces; these surfaces however, such as the ventricular surface, cover only a small portion of nervous tissue. The achievement of optimal preserved internal regions of the nervous system and the observation of not naturally occurring surfaces is still the object of SEM methodological efforts. The present accomplishments of the SEM technique are not restricted by the resolution power of the microscope but rather by the unsolved problems concerning the preparation of specimens.


Lateral Ventricle Chick Embryo Area Postrema Olfactory Mucosa Tectorial Membrane 
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  • K. Meller

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