Inhibition of Hematopoietic Cell Proliferation
In healthy post-natal human beings, hematopoiesis is restricted to the bone marrow. As mature blood cells are utilized or die in the periphery, they are continuously replenished by the bone marrow. For years, it has been recognized that blood cell renewal is accomplished by maturation of morphologically identifiable precursors (blast cells) in the bone marrow. More recently, with the development of hematopoietic cell culture techniques, the concept has evolved that these morphologically recognizable precursor cells are in turn replenished from hematopoietic stem cells. In this hierarchical scheme of hematopoiesis, hematopoietic stem cells have the dual capacities of self-renewal and differentiation; that is, they are capable both of maintaining their own numbers and of giving rise to progeny committed to a single line of differentiation. A model of hematopoiesis based on this scheme is depicted in Fig. 1.
KeywordsColony Formation Aplastic Anemia Chronic Myelogenous Leukemia Human Bone Marrow Bone Marrow Failure
Unable to display preview. Download preview PDF.
- Appelbaum, F.R., Fefer, A., Cheever, M.A., Hansen, J., Greenberg, P.D., Thomas, E.D.: Treatment of aplastic anemia with identical twin bone marrow transplantation. Blood (Suppl. 1)54, 227 a (1979).Google Scholar
- Freedman, M.H., Saunders, E.F.: Diamond-Blackfan syndrome. Clin. Res. 25, 339 A (1977).Google Scholar
- Gale, R.P.: Bone marrow transplantation in identical twins with aplastic anemia. Blood (Suppl.) 54, 229 a (1979).Google Scholar
- Iscove, N.N., Sieber, F., Winterhalter, K.H.: Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirements for erythropoietin by gel formation and affinity chromatography on agarose-concanavalin A. J. Cell. Physiol. 83, 309–320 (1974).PubMedCrossRefGoogle Scholar
- Knudtzon, S., Mortensen, B.T.: Interaction between normal and leukaemic human cells in agar culture. Br. J. Haematol. 17, 369–378 (1976).Google Scholar
- Laerum, O.D., Maurer, H.R.: Proliferation kinetics of myelopoietic cells and macrophages in diffusion chambers after treatment with granulocyte extracts (chalone). Virchows Arch. (Cell Pathol.) 14, 293–305 (1973).Google Scholar
- Metcalf, D., Moore, M.A.S.: Hematopoietic cells. in: Frontiers in biology. Neuberger, A., Tatum, E.L. (eds.), p. 109. Amsterdam, London: North-Holland 1971.Google Scholar
- Nakeff, A., Daniels-McQueen, S.: In vitro colony assay for a new class of megakaryocyte precursor: colony-forming unit megakaryocyte (CFU-M): Proc. Soc. Exp. Biol. Med. 151, 587–590 (1976).Google Scholar
- Rosenszajn, L.A., Shoham, D., Kalechman, I.: Clonal proliferation of PHA-stimulated human lymphocytes in soft agar culture. Immunology 21, 1041–1055 (1975).Google Scholar
- Singer, J.W., Brown, J.E., James, M.C., Doney, K., Warren, R.P., Storb, R., Thomas, E.D.: Effect of peripheral blood lymphocytes from patients with aplastic anemia or granulocyte colony growth from HLA-matched and-mismatched marrow: Effect of transfusion sensitization. Blood 52, 37–46 (1978).PubMedGoogle Scholar
- Speck, B., Gluckman, E., Haak, H.L., Rood, J.J. van: Treatment of aplastic anemia by an-tilymphocyte globulin with and without allogeneic bone marrow infusions. Lancet 1977 II, 1145–1148.Google Scholar
- Torok-Storb, B., Storb, R., Thomas, E.D.: In vitro studies of 35 patients with aplastic anemia: Correlation with marrow graft failure or graft rejection. Blood (Suppl. 1) 54, 231a (1979).Google Scholar