Cell Fusion as a Tool for the Detection of Viral Footprints in Childhood Leukemia
In our studies on a possible role of type C oncoviruses in human leukemia we applied the technique of cocultivation of human bone marrow with an animal indicator cell line. Dog thymus A7573 cells were cocultivated with human bone marrow samples from normal individuals, leukemic patients, and nonleukemic patients. By means of the indirect cytoplasmic immunofluorescence assay (IFA), antigens which crossreacted with the major internal protein (p30) of the woolly monkey (simian) sarcoma leukemia virus (SiSV) complex could be detected (Nooter et al. 1979). In the case of childhood leukemia, five out of nine cocultures showed virus-related IFA staining.
KeywordsCell Fusion Human Bone Marrow Childhood Leukemia Woolly Monkey Nucleate Bone Marrow Cell
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- Joustra M, Lundgren H (1970) Preparation of freeze-dried monomeric and immunochemically IgG by a rapid and reproducible technique. In: Peeter H (ed) Protides of the biological fluid. Pergamon Oxford, p 511Google Scholar
- Nooter K (1979) Studies on the role of RNA tumour viruses in human leukaemia. Thesis, University of Leiden, pp 38–40Google Scholar
- Szybalski W, Szybalska EH, Ragni G (1962) Genetic studies with human cell lines. Natl Cancer Inst Monogr 7:75Google Scholar