Cell Fusion as a Tool for the Detection of Viral Footprints in Childhood Leukemia
In our studies on a possible role of type C oncoviruses in human leukemia we applied the technique of cocultivation of human bone marrow with an animal indicator cell line. Dog thymus A7573 cells were cocultivated with human bone marrow samples from normal individuals, leukemic patients, and nonleukemic patients. By means of the indirect cytoplasmic immunofluorescence assay (IFA), antigens which crossreacted with the major internal protein (p30) of the woolly monkey (simian) sarcoma leukemia virus (SiSV) complex could be detected (Nooter et al. 1979). In the case of childhood leukemia, five out of nine cocultures showed virus-related IFA staining.
KeywordsLeukemia Polyethylene Glycol Sedimentation Sarcoma
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