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Identification of the Avian Myeloblastosis Virus Genome

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Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY,volume 26)

Abstract

In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute myeloblastic leukemia is induced by a defective leukemogenic component. To identify the leukemogenic viral genome the unintegrated and integrated viral DNA intermediates were chracterized. Linear viral DNA isolated from the cytoplasm of helper virus (MAV-1 or MAV-2) infected chicken embryonic fibroblasts (CEF) has a mass of 5.3 million daltons (md) (Bergmann et al. 1980). The linear MAV-1 and MAV-2 DNAs can be distinguished from one another by cleavage with the restriction endonuclease Hind III, because MAV-1 DNA contains one more Hind III recognition site than does MAV-2. Linear viral DNA isolated from the cytoplasm of CEF infected with standard AMV (AMV-S) which contains the defective leukemogenic component showed a minor molecular species of 4.9 md in addition to the major species of 5.3 md. Eco RI or Hind III digestion of the linear viral DNA from AMV-S-infected CEF generates specific fragments in addition to those unique for MAV-1 or MAV-2 viral DNA (Bergmann et al. 1980). A Hind III digest of AMV-S linear viral DNA also indicates that more than 90% of the AMV-S virus complex is MAV-1 like, while the remainder represents both the leukemogenic and MAV-2-like viruses (Souza et al. 1980).

Keywords

  • Acute Myeloblastic Leukemia
  • Helper Virus
  • Avian Myeloblastosis Virus
  • Proviral Sequence
  • Chicken Embryonic Fibroblast

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  • Bergmann DG, Souza LM, Baluda MA (1980) Characterization of the avian myeloblastosis associated virus DNA intermediates. J Virol 34:366–372

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  • Souza LM, Baluda MA (to be published) Identification of the avian myeloblastosis virus genome. I. Identification of restriction endonuclease fragments associated with acute myeloblastic leukemia. J Virol

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  • Souza LM, Komaromy MC, Baluda MA (1980) Identification of a proviral genome associated with avian myeloblastic leukemia. Proc Natl Acad Sci USA 77:3004–3008

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  • Souza LM, Strommer JN, Hillyard RL, Komaromy MC, Baluda MA (to be published b) Cellular sequences are present in the presumptive avian myeloblastosis virus genome. Proc Natl Acad Sci USA

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© 1981 Springer-Verlag Berlin Heidelberg

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Souza, L.M., Bergmann, D.G., Baluda, M.A. (1981). Identification of the Avian Myeloblastosis Virus Genome. In: Neth, R., Gallo, R.C., Graf, T., Mannweiler, K., Winkler, K. (eds) Modern Trends in Human Leukemia IV. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67984-1_78

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  • DOI: https://doi.org/10.1007/978-3-642-67984-1_78

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-10622-7

  • Online ISBN: 978-3-642-67984-1

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