Summary
In the last 3 years we were able to establish four long-term cultures from Hodgkin-derived material [pleuraleffusions (2), bone marrow (1), and peripheral blood (1)], consisting of cells which represent morphologic and cytochemical as well as cytogenetic features of their in vivo ancestors. Two of these cell lines are described in this paper.
These two lines share the same features: non-B-T lymphocytes, non-macrophages, non myeloid cells, EBV genome negative, monoclonality, multiple numerical and structural chromosome aberrations, and tumor formation upon intracranial xenotransplantation in nude mice.
The two remaining lines are being characterized at the moment. The common characteristics expressed synonymously in the two described lines suggest that the Hodgkin tumor cell does not seem to share the features of marker-carrying lymphocytes, macrophages, or myeloblasts. The cellular origin of these cells is not clear. The loss of cellular differential markers during the process of possible dedifferentiation is discussed.
This work was supported by the Deutsche Forschungsgemeinschaft Bad Godesberg (Di 184-5/6)
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Diehl V, Krause P, Hellriegel KP, Busche M, Schedel J, Laskewitz E (1977) Lymphoid cell lines: in vitro cell markers in correlation to tumorigenicity in nude mice. In: Thierfelder S, Rodt H, Thiel E (eds) Haematology and blood transfusion: Immunological diagnosis of leukemias and lymphomas, vol 20. Springer, Berlin Heidelberg New York pp 289–296
Dorfman RF, Rice DF, Mitchel AD, Kempson RL, Levine G (1973) Ultrastructural studies of Hodgkin’s disease. Natl Cancer Inst Monogr 36:221–238
Hellriegel KP, Diehl V, Krause PH, Meider S, Blankenstein M, Busche W (1977) The significance of chromosomal findings for the differentiation between lymphoma and lymphoblastoid cell lin es. In: Thierfelder S, Rodt H, Thiel E (eds) Haematology and blood transfusion: Immunological diagnosis of leukemias and lymphomas, Vol 20. Springer, Berlin Heidelberg New York, p 307
Henle G, Henle W (1966) Immunofluorescence in cells derived from Burkitt’s lymphoma. J Bacteriol 91:1248–1256
Jondal M, Holm G, Wigzell H (1972) Surface markers on human T- and B-cells. J Exp Med 136:207
Krause P, Schmitz R, Lindemann M, Georgii A (1975) Xenotransplantation etablierter Tumorzellen auf congenital thymuslose “nude”-Mäuse. Z Krebsforsch 83:177
Lozzio BB, Lozzio CB, Machado E (1976) Brief Communication: Human myelogenous (Ph 1 +) leukemia cell line: Transplantation into athymic nude mice. J Natl Cancer Inst 56 3:627–629
Mori Y, Lennert K (1969) Electron microscopic atlas of lymph node cytology and pathology. Springer, Berlin Heidelberg New York, pp 29–30
Papadimitriou CS, Stein H, Lennert K (1978) The complexity of immunohistochemical staining pattern of Hodgkin and Sternberg-Reed cells
Demonstration of Immunoglobulin, albumin α1antichymotrypsin and lysozyme. Int J Cancer 21:531–541
Rappaport H (1966) Tumors of the hematopoetic system. Atlas of tumor pathology Sect 3, Fasc 8. Armed Forces Institute of Pathology, Washington DC
Reedman BM, Klein G (1973) Cellular localisation of an Epstein-Barr virus (EBV)-associated complementfixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer 11:499–520
Schaadt M, Kirchner HH, Fonatsch Ch, Diehl V (1979) Intracranial heterotransplantation of human hematopoetic cells in nude mice. Int J Cancer 23:751–761
Sumner AT, Evans HJ, Buckland RA (1971) New technique for distinguishing between human chromosomes. Nature [New Biol] 232:31–32
Stein H (1978) The immunologic and immunochemical basis for the Kiel classification. In: Lennert K (ed) Malignant lymphomas other than Hodgkin’s disease. Springer, Berlin Heidelberg New York (Handbuch der speziellen pathologischen Anatomie und Histologic, vol I/3B, pp 529–657)
Stein H, Kaiserling E (1974) Surface immunoglobulins and lymphocyte — specific antigens on leukemic reticuloendotheliosis cells. Clin Exp Immunol 18:63–71
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Diehl, V., Kirchner, H.H., Schaadt, M., Fonatsch, C., Stein, H. (1981). Lymphoproliferation and Heterotransplantation in Nude Mice: Tumor Cells in Hodgkin’s Disease. In: Neth, R., Gallo, R.C., Graf, T., Mannweiler, K., Winkler, K. (eds) Modern Trends in Human Leukemia IV. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67984-1_38
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