Abstract
Analysis and sorting of chromosomes using fluorescence-based high speed flow systems was reported in 1975 (Gray et al. 1975a). In the ensuing 5 years these principles have been applied to karyotype analysis (Gray et al. 1975b: Stubblefield et al. 1975: Carrano et al. 1976, 1978, 1979a,b; Otto and Oldiges 1978; Gray et al., 1979) and to chromosome biochemistry and cytochemistry (Sawin et al. 1979; Lebo et al. 1979; Langlois et al. 1980). The results of these investigations indicate that the use of these systems for chromosome analysis and sorting is an important adjunct to existing cytogenetic techniques and a primary resource for molecular cytogenetic studies of individual chromosomes. This brief report describes one application of this approach, namely, regional gene mapping of human chromosomes.
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References
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Carrano, A.V., Lebo, R.V., Yu, L.C., Kan, Y.W. (1981). Regional Gene Mapping of Human Chromosomes Purified by Flow Sorting. In: Neth, R., Gallo, R.C., Graf, T., Mannweiler, K., Winkler, K. (eds) Modern Trends in Human Leukemia IV. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67984-1_25
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DOI: https://doi.org/10.1007/978-3-642-67984-1_25
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