Skip to main content
SpringerLink
Log in

Weitere EM-Techniken

  • Chapter
Elektronenmikroskopische Laborpraxis

  • 63 Accesses

Zusammenfassung

Hier sollen der Vollständigkeit halber einige Techniken vorgestellt werden, die in der Medizin/Zellbiologie derzeit keine Bedeutung haben, sondern die Auflösungsgrenze des Lichtmikroskops für die Morphologie und zum Teil auch Anatomie durchbrachen. Am wichtigsten von diesen Techniken ist die Raster- oder Scanning-Elektronenmikroskopie, doch soll vorerst noch ein TEM-Verfahren kurz erwähnt werden.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 49.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 74.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literaturverzeichnis

Handbücher, Serien und wichtige Monographien

  • Flickinger CJ (1979) Medical cell biology. WB Saunders, Philadelphia, PA

    Google Scholar 

  • Glauert AM (ed) (ab 1972 ) Practical methods in electron microscopy. North-Holland, Amsterdam

    Google Scholar 

  • Gray P (ed) (1973) The encyclopedia of microscopy and microtechnology. Van Nostrand Reinhold Company, New York

    Google Scholar 

  • Hayat MA (ed) (ab 1970 ) Principles and techniques of electron microscopy. Van Nostrand Reinhold Company, New York

    Google Scholar 

  • Hayat MA (ed) (ab 1974 ) Principles and techniques of scanning electron microscopy. Van Nostrand Reinhold Company, New York

    Google Scholar 

  • Koehler JK (ed) Advanced techniques in biological electron microscopy. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Ledbetter MC, Porter KR (1970) Introduction to the fine structure of plant cells. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Porter KR, Bonneville MA (1965) Einführung in die Feinstruktur von Zellen und Geweben. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Reimer L (1967) Elektronenmikroskopische Untersuchungs-und Präparationsmethoden, 2. Aufl. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Reimer L, Pfefferkorn G (1977) Raster-Elektronenmikroskopie, 2. Aufl. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Ruthmann A (1966) Methoden der Zellforschung. Franckh/Kosmos, Stuttgart

    Google Scholar 

  • Schimmel G, Vogell W (eds) (ab 1973 ) Methodensammlung der Elektronenmikroskopie. Wissenschaftliche Verlagsanstalt, Stuttgart

    Google Scholar 

Atlanten

  • Dodge JD (1968) An atlas of biological ultrastructure. Edward Arnold, London

    Google Scholar 

  • Fawcett DW (1966) An atlas of fine structure. The cell. WB Saunders, Philadephia London

    Google Scholar 

  • Kessel RG, Shih CY (1974) Scanning electron microscopy. A student’s atlas on biological organization. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Krstie RV (1976) Ultrastruktur der Säugetierzelle. Ein Atlas zum Studium für Mediziner und Biologen. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Krstié RV (1978) Die Gewebe des Menschen und der Säugetiere. Ein Atlas zum Studium für Mediziner und Biologen. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Rhodin JAG (1974) Histology. A text, an atlas. Oxford University Press, New York

    Google Scholar 

  • Sanga F (1964) Atlas of electron microscopy. Biological application. Elsevier, Amsterdam

    Google Scholar 

  • Threadgold LT (1976) The ultrastructure of the animal cells, end edn. Pergamon Press, Oxford

    Google Scholar 

Zitierte Veröffentlichungen

  • Amlacher E (1974) Autoradiographie in Histologie und Cytologie. G Fischer, Stuttgart

    Google Scholar 

  • Bahr GF (1973) Determination of the dry mass of small biological objects by quantitative electron microscopy. In: Heuhoff V (ed) Micromethods in molecular biology. Springer, Berlin Heidelberg New York, pp 257–284

    Google Scholar 

  • Beaulaton J, Gras R (1980) Influence of aldehyde fixatives on the ultrastructure of the prothoracic gland cells in Rhodnius prolixus ( Insecta, Heteroptera) with special reference to their osmotic effects. Microsc Acta 82: 351–366

    Google Scholar 

  • Bernhard W (1969) A new staining procedure for electron microscopical cytology. J Ultrastruct Res 27: 250–265

    Article  Google Scholar 

  • Bienz KA (1977) Techniques and applications of autoradiography in the light and electron microscope. Micros Acta 79: 1–22

    Google Scholar 

  • Carling DE, White JA, Brown MF (1977) The influence of fixation procedure on the ultrastructure of the host-endophyte interface of vesicular-arbuscular mycorrhizae. Can J Bot 55: 48–51

    Article  Google Scholar 

  • Chandra P, Appel W (1973) Methoden der Molekularbiologie. G Fischer, Stuttgart

    Google Scholar 

  • Egger D, Bienz K (1975) A method for distinction between RNA and DNA in aldehyde and osmiumtetroxide-fixed electron microscopic autoradiographs. Experientia 31: 1371–1373

    Article  Google Scholar 

  • Fischer HA (1979) High resolution scintiautoradiography. J Histochem Cytochem 27: 1527–1528

    Article  Google Scholar 

  • Fuge H (1980) Microtubule disorientation in anaphase half-spindles during autosome segregation in crane fly spermatocytes. Chromosoma 76: 309–328

    Article  Google Scholar 

  • Gaffal KP, Kreutzer D (1977) The mitochondria of Polytoma papillatum at two different stages of the vegetative cell cycle. Protoplasma 91: 167–177

    Article  Google Scholar 

  • Gautier A, Cogliati R, Schreyer M, Fakan J (1973) “Feulgen-type” and “PAS-type” reactions at the ultrastructural level. In: Wisse E, Deams WTh, Molenaar I, van Duijn P (eds) Electron microscopy and cytochemistry. North-Holland, Amsterdam, pp 271–274

    Google Scholar 

  • Geyer G (1973) Ultrahistochemie, 2. Aufl. G Fischer, Stuttgart

    Google Scholar 

  • Gicquaud C, Gruda J, Pollender J-M (1980) La phalloidine protège la F-actine contre les effets destructeurs de l’acide osmique et du permanganate. Eur J Cell Biol 20: 234–239

    Google Scholar 

  • Goodhew PJ, Chescoe D (1980) Microanalysis in the transmission electron microscope. Micron 11: 153–182

    Google Scholar 

  • Gori P (1978) Ponceau 2R staining of proteins and periodic acid bleaching of osmicated subcellular structures on semi-thin sections of tissues processed for electron microscopy: a simplified procedure. J Microsc 114: 111–113

    Article  Google Scholar 

  • Hall JL (ed) (1978) Electron microscopy and cytochemistry of plant cells. Elsevier/ North-Holland, Amsterdam

    Google Scholar 

  • Hawkes PW (ed) (1980) Computer processing of electron microscopic images. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Hayat MA (ed) (1975) Electron microscopy of enzymes, vol IV. Principles and methods. Van Nostrand Reinhold, New York

    Google Scholar 

  • Heuser JE, Salpeter SR (1979) Organization of acetylcholine receptors in quick-frozen, deet-etched, and rotary-replicated Torpedo postsynaptic membrane. J Cell Biol 82: 150–173

    Article  Google Scholar 

  • Kam Z (1980) The reconstruction of structure from electron micrographs of randomly oriented particles. J Theor Biol 82: 15–39

    Article  Google Scholar 

  • Klein H-P, Stockem W (1976) Präparation biologischer Objekte für das Raster-Elektronenmikroskop. Microsc Acta 78: 388–406

    Google Scholar 

  • Kleinschmidt AK, Lang D, Zahn RK (1960) Darstellung molekularer Fäden von DNS. Naturwissenschaften 47: 96

    Article  Google Scholar 

  • Kopriwa BM (1975) A comparison of various procedures for fine grain development in electron microscopic autoradiography. Histochemistry 44: 201–224

    Article  Google Scholar 

  • Kopriwa BM (1979) Examination of various methods for the intensification of radio-autographs by scintillators. J Histochem Cytochem 27: 1524–1526

    Article  Google Scholar 

  • Ladhoff A-M (1980) Über Möglichkeiten der elektronenoptischen Strukturanalyse von hochmolekularen Ribonukleinsäuren. Biol Zentralbl 99: 385–397

    Google Scholar 

  • Langenberg WG (1978) Relative speed of fixation of glutaraldehyde and osmic acid in plant cells measured by grana appearance in chloroplasts. Protoplasma 94: 167–173

    Article  Google Scholar 

  • Lima-de-Faria A (1974) The three-dimensional organization of the amplified rDNA complex in Acheta. Proc Natl Acad Sci USA 71: 4778–4782

    Article  ADS  Google Scholar 

  • Lima-de-Faria A, Pero R, Avanzi S, Durante M, Stehle U, D’Amato F, Granström H (1975) Relation between rRNA genes and the DNA satellites of Phaseolus coccineus. Hereditas 79: 5–20

    Article  Google Scholar 

  • Lutz C, Nagl W (1980) A reliable method for preparation and electron microscopic visualization of nucleosomes in higher plants. Planta 149: 408–410

    Article  Google Scholar 

  • Miller OL, Beatty BR (1969) Portrait of a gene. J Cell Physiol 74, Suppl 1: 225–232

    Article  Google Scholar 

  • Miwa T, Takanami M, Yamagishi H (1979) Electron microscopic visualization of restriction sites on DNA molecules. Gene 6: 319–330

    Article  Google Scholar 

  • Mollenhauer HH (1964) Plastic embedding mixtures for use in electron microscopy. Stain Technol 39: 111–114

    Google Scholar 

  • Moor H, Mühlethaler K (1963) Fine structure in frozen-etched yeast cells. J Cell Biol 17: 609–628

    Article  Google Scholar 

  • Moyne G (1980) Methods in ultrastructural cytochemistry of the cell nucleus. G Fischer, Stuttgart

    Google Scholar 

  • Nagl W (1977) Nuclear structures during cell cycles. In: Rost TL, Gifford EM jr (eds) Mechanisms and control of cell division. Dowden, Hutchinson & Ross, Stroudsburg, PA, pp 147–193

    Google Scholar 

  • Nagl W (1979) Nuclear ultrastructure: Condensed chromatin in plants is species-specific (karyotypical), but not tissue-specific (functional). Protoplasma 100: 53–71

    Article  Google Scholar 

  • Nagl W (1980) Species and hybrid diagnosis in plants by means of quantitative light and electron microscopic morphometry of chromatin texture. Microsc Acta Suppl 4: 19–25

    Google Scholar 

  • Nagl W (1980) Species and hybrid diagnosis in plants by means of quantitative light and electron microscopic morphometry of chromatin texture. Microsc Acta Suppl 4: 19–25

    Google Scholar 

  • Nicolini C (1980) Nuclear morphometry, quinternary chromatin structure and cell growth. J Submicrosc Cytol 12: 475–505

    Google Scholar 

  • Paweletz N (1967) Präparationsmethoden für die elektronenmikroskopische Autoradiographie. Siemens Mitt Eg 1 /201

    Google Scholar 

  • Pressman NJ, Wied GL (eds) (1979) The automation of cancer cytology and cell image analysis. Tutorials of Chicago, IL

    Google Scholar 

  • Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17: 208–212

    Article  Google Scholar 

  • Rittenburg JH, Bayer RC, Gallagher ML, Leavitt DF (1979) A rapid technique for preparing microorganisms for transmission electron microscopy. Stain Technol 54: 275–280

    Google Scholar 

  • Robards AW (1978) An introduction to techniques for scanning electron microscopy of plant cells. In: Hall JL (ed) Electron microscopy and cytochemistry of plant cells. Elsevier/North-Holland, Amsterdam, pp 343–415

    Google Scholar 

  • Sabatini DD, Bensch K, Burnett RJ (1963) The preservation of cellular ultrastructure and enzymatic activity by cytochemistry and electron microscopy. J Cell Biol 17: 19–58

    Article  Google Scholar 

  • Salema R, Brandao I (1973) The use of PIPES buffer in the fixation of plant cells for electron microscopy. J Submicrosc Cytol 5: 79–96

    Google Scholar 

  • Sandritter W, Riede U, Kiefer G (1978) Versuche zur Quantifizierung des Chromatins im Elektronenmikroskop. Acta Histochem Suppl 20: 203–209

    Google Scholar 

  • Schiller A, Taugner R (1980) Freeze-fracturing and deep-etching with the volatile cryoprotectant ethanol reveals true membrane surfaces of kidney structures. Cell Tissue Res 210: 57–69

    Article  Google Scholar 

  • Schroeder HE, Rossinsky K, Müller W (1980) An established routine method for differential staining of epoxy-embedded tissue sections. Microsc Acta 83: 111–116

    Google Scholar 

  • Sjöstrand FS (1979) The interpretation of pictures of freeze-fractured biological material. J Ultrastruct Res 69: 378–420

    Article  Google Scholar 

  • Sleytr UB, Robards AW (1977) Freeze-fracturing: a review of methods and results. J Microsc 111: 77–100

    Article  Google Scholar 

  • Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26: 31–43

    Article  Google Scholar 

  • Stockert JC, Colman OD (1978) Toluidine blue staining on epon smithin sections: effects of an oil differentiation. Microsc Acta 80: 97–105

    Google Scholar 

  • Takagi I, Sato T, Yamada K (1979) A rapid method for embedding fractionated samples for electron microscopy. J Electron Microsc 28: 316–319

    Google Scholar 

  • Thoma F, Koller Th, Klug A (1979) Involvement of histone Hl in the Organization of the nucleosome and of the salt-dependent superstructures of chromatin. J Cell Biol 83: 403–427

    Article  Google Scholar 

  • Trendelenburg MF (1974) Morphology of rRNA cistrons in oocytes of the water beetle, Dytiscus marginalis L. Chromo soma 48: 119–135

    Google Scholar 

  • Wells R, Ingle J (1970) The constancy of the buoyant density of chloroplast and mitochondrial DNA’s in a range of higher plants. Plant Physiol 46: 178–179

    Article  Google Scholar 

  • Williams MA (1977) Autoradiography and immunocytochemistry, and quantitative methods in biology, vol VI. In: Glauert AM (ed). North-Holland, Amsterdam

    Google Scholar 

  • Williams RC (1977) Use of polylysine for adsorption of nucleic acids and enzymes to electron microscopic specimen films. Proc Natl Acad Sci USA 74: 2311–2315

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. FB Biologie, Universität Kaiserslautern, Postfach 30 49, 6750, Kaiserslautern, Deutschland

    Professor Dr. Walter Nagl

Authors
  1. Professor Dr. Walter Nagl
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1981 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Nagl, W. (1981). Weitere EM-Techniken. In: Elektronenmikroskopische Laborpraxis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67976-6_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-67976-6_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-10615-9

  • Online ISBN: 978-3-642-67976-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation