Characterization of the Nitrite-Oxidizing System in Nitrobacter

  • H. Sundermeyer
  • E. Bock
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Nitrobacter X 14 was isolated from soil of the old Botanical Garden in Hamburg. It is a facultative lithotrophic nitrite-oxidizing bacterium. As well as Nitrobacter agilis (Smith and Hoare, 1968; Bock, 1976) Nitrobacter X 14 was able to grow mixotrophically and heterotrophically. In contrast to other strains of Nitrobacter the doubling time of autotrophically grown cells was not 13 h but 40 h. After nitrite consumption the cell yield with 3 mg protein/1 was lower than in Nitrobacter agilis (5.5 mg protein/1). Under mixotrophic conditions with nitrite, pyruvate, and yeast extract-peptone (py—ni cells) growth was much better. The doubling time was 10– 11 h and the cell yield amounted to 17 mg protein/1 when the nitrite was consumed. When Nitrobacter X 14 was grown heterotrophically with pyruvate as carbon and energy source and yeast extract-peptone to meet nitrogen requirements (y—py cells) cells doubled within 15– 20h, when grown with pyruvate and casamino acids (cas—py cells) the doubling time increased to more than 20 h. Compared to Thiobacillus intermedius (London and Rittenberg, 1966) Nitrobacter X 14 should be regarded as an organism highly specialized to mixotrophic growth.


Membrane Fraction Casamino Acid Nitrite Oxidation Mixotrophic Growth Intracytoplasmic Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



adenosine triphosphatase




cytoplasmic membrane


intracytoplasmic membrane


poly-β-hydroxybutric acid


ribulose-1,5-bisphosphate carboxylase


sodium dodecylsulfate

py—ni cells

cells grown mixotrophically with nitrite, pyruvate and yeast extract-peptone

cas—py cells

cells grown he terotrophically with pyruvate and casamino acids

y—py cells

cells grown heterotrophically with pyruvate and yeast extract-peptone


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abrams A, McNamara T, Johnson BF (1960) J Biol Chem 235: 3659–3662Google Scholar
  2. Aleem MJH (1968) Biochim Biophys Acta 162: 338–347PubMedCrossRefGoogle Scholar
  3. Aleem MJH, Hoch GE, Varner JE (1965) Proc Natl Acad Sei USA 54: 869–873CrossRefGoogle Scholar
  4. Bock E (1976) Arch Microbiol 108: 305–312PubMedCrossRefGoogle Scholar
  5. Davies PL, Bragg PD (1972) Biochim Biophys Acta 266: 273–284PubMedCrossRefGoogle Scholar
  6. Fauü KF, Wallaee W, Nicholas DJD (1969) Biochim J 113: 449–455Google Scholar
  7. London J, Rittenberg SC (1966) J Bacteriol 91: 1062–1069PubMedGoogle Scholar
  8. O’Kelley JC, Becker GE, Nason A (1970) Biochim Biophys Acta 205: 409–425PubMedCrossRefGoogle Scholar
  9. Shively JM, Bock E, Westphal K, Cannon GC (1977) J Bacteriol 132: 673–675PubMedGoogle Scholar
  10. Smith AJ, Hoare DS (1968) J Bacteriol 95: 844–855PubMedGoogle Scholar
  11. Tsien HC, Laudelout H (1968) Arch Microbiol 61: 280–291Google Scholar
  12. Watson SW, Mandel M (1971) J Bacteriol 107: 563–569PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • H. Sundermeyer
    • 1
  • E. Bock
    • 1
  1. 1.Institut für Allgemeine Botanik, MikrobiologieUniversität HamburgGermany

Personalised recommendations