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Characterization of the Nitrite-Oxidizing System in Nitrobacter

  • H. Sundermeyer
  • E. Bock
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Nitrobacter X 14 was isolated from soil of the old Botanical Garden in Hamburg. It is a facultative lithotrophic nitrite-oxidizing bacterium. As well as Nitrobacter agilis (Smith and Hoare, 1968; Bock, 1976) Nitrobacter X 14 was able to grow mixotrophically and heterotrophically. In contrast to other strains of Nitrobacter the doubling time of autotrophically grown cells was not 13 h but 40 h. After nitrite consumption the cell yield with 3 mg protein/1 was lower than in Nitrobacter agilis (5.5 mg protein/1). Under mixotrophic conditions with nitrite, pyruvate, and yeast extract-peptone (py—ni cells) growth was much better. The doubling time was 10– 11 h and the cell yield amounted to 17 mg protein/1 when the nitrite was consumed. When Nitrobacter X 14 was grown heterotrophically with pyruvate as carbon and energy source and yeast extract-peptone to meet nitrogen requirements (y—py cells) cells doubled within 15– 20h, when grown with pyruvate and casamino acids (cas—py cells) the doubling time increased to more than 20 h. Compared to Thiobacillus intermedius (London and Rittenberg, 1966) Nitrobacter X 14 should be regarded as an organism highly specialized to mixotrophic growth.

Keywords

Membrane Fraction Casamino Acid Nitrite Oxidation Mixotrophic Growth Intracytoplasmic Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ATPase

adenosine triphosphatase

cyt.

cytochrome

CM

cytoplasmic membrane

ICM

intracytoplasmic membrane

PHB

poly-β-hydroxybutric acid

RuBPCase

ribulose-1,5-bisphosphate carboxylase

SDS

sodium dodecylsulfate

py—ni cells

cells grown mixotrophically with nitrite, pyruvate and yeast extract-peptone

cas—py cells

cells grown he terotrophically with pyruvate and casamino acids

y—py cells

cells grown heterotrophically with pyruvate and yeast extract-peptone

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Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • H. Sundermeyer
    • 1
  • E. Bock
    • 1
  1. 1.Institut für Allgemeine Botanik, MikrobiologieUniversität HamburgGermany

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