Enzymatic Mechanism and Regulation of Nitrate Reduction in Rhodopseudomonas capsulata
The ability to assimilate nitrate as N-souree is widespread among prokaryotes. When studying a strain of the facultative phototrophic bacterium, Rhodospirillum (R.) rubrum, Katoh (1963 a) found that the bacteria could be adapted to grow with nitrate as sole N-source under photosynthetic conditions or with nitrate as terminal electron acceptor under dark-anaerobic conditions. The nitrate reductase from such adapted cells was partially purified and characterized as a cytochrome b-containing enzyme complex (Katoh, 1963b). Taniguchi and Kamen (1963) obtained growth of the same R. rubrum strain (S1) with nitrate as N-source both under photosynthetic and aerobic conditions. Contrary to the results of the latter authors, we were unable to grow any of four different R. rubrum strains (including strain S1) with nitrate as sole N-source under photosynthetic conditions in defined media with D,L-malate as C-source. In our laboratory, among 35 strains of the more common species of the Rhodospirillaceae, the capacity of assimilatory nitrate reduction was restricted to 11 strains of the two taxonomically related speciesRhodopseudomonas (Rps.) capsulata and Rps. Sphaeroides (Klemme, 1979).
KeywordsMutant Strain Nitrate Reductase Nitrate Reductase Activity Rhodotorula Glutinis Nitrite Reductase Activity
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