In Vitro Sensitization of Rat Mast Cells by Human Serum IgE Tested by Histamine Release

  • R. Sonak
Conference paper


A successful sensitization of rat mast cells (RMC) by human IgE (HuIgE) was first reported by Perelmutter and Khera [1]. In later experiments, Perelmutter and his collaborators confirmed these results [2–4] and other investigators also published positive results [5–7]. Nevertheless, Plaut et al. [8] were not able to sensitize RMC with HuIgE. The problem has still not been unambiguously solved.


Histamine Release Histamine Liberation Vitro Sensitization Successful Sensitization Echinococcus Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Perelmutter L, Khera K (1970) A study on the detection of human reagins with rat peritoneal mast cells. Int Arch Allergy Appl Immunol 39:27–44PubMedCrossRefGoogle Scholar
  2. 2.
    Perelmutter L, Liakopoulou A (1974) Interrelationship of human IgE antibody and allergen in the rat mast cell technique. Int Arch Allergy Appl Immunol 46:280–288PubMedCrossRefGoogle Scholar
  3. 3.
    Phills JA, Perelmutter L (1974) IgE mediated and non-IgE mediated allergic-type reactions to aspirin. Acta Allergol (Copenh) 29:474–490Google Scholar
  4. 4.
    Pontefract R, Perelmutter L (1976) An electron microscope study of receptor sites on rat mast cells for human Immunoglobulin E. Acta Allergol (Copenh) 31:184–192Google Scholar
  5. 5.
    Haddad ZH, Lopapa A, Korotzer J (1971) Direct demonstration of human Immunoglobulin E (IgE) on rat mast cells after passive sensitization in vitro. J Allergy 47:88Google Scholar
  6. 6.
    Vijayanagar HM, Attallah NA, David MF, Sehon AH (1974) Detection of human reagins with rat peritoneal mast cells by histamine release. Int Arch Allergy Appl Immunol 46:375–392PubMedCrossRefGoogle Scholar
  7. 7.
    Renoux ML, Montis G de, Roche A, Marcelli D (1975) Binding of human IgE Immunoglobulin to rat mast cells. Int Arch Allergy Appl Immunol 49:478–490PubMedCrossRefGoogle Scholar
  8. 8.
    Plaut M, Lichtenstein LM, Bloch KJ (1973) Failure to obtain histamine release from rat mast cells exposed to human allergic serum and specific antigen or IgE myeloma protein and anti IgE. J Immunol 111:1022–1030PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • R. Sonak
    • 1
  1. 1.Institut für Hygiene der Universität MünsterGermany

Personalised recommendations