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Cell Density Profile as a Measure of Erythrocyte Hydration: Therapeutic Alteration of Salt and Water Content in Normal and SS Red Blood Cells

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Automation in Hematology

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Abstract

The incubation of red blood cells in high concentrations of sodium bicarbonate produces a net influx of salt and water resulting in the dilution of cell hemoglobin. After reinfusion into the donor, cells swollen in this manner can be readily identified in peripheral blood samples by their low density on phthalate gradients. It is proposed that this manipulation of cell water content may have therapeutic implications for sickle cell disease, since the rate of deoxyhemoglobin S gelation is retarded by small reductions in hemoglobin concentration.

A portion of this work has been published in abstract form: Clin. Res. 27, 463A, 1979

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Authors and Affiliations

  1. Division of Hematology, Departments of Medicine and Laboratory Medicine, School of Medicine, The University of North Carolina, 27514, Chapel Hill, North Carolina, USA

    Eugene P. Orringer, Marjorie E. S. Roer & John C. Parker

Authors
  1. Eugene P. Orringer
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  2. Marjorie E. S. Roer
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  3. John C. Parker
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Editor information

Editors and Affiliations

  1. University of North Carolina, School of Medicine, 27514, Chapel Hill, NC, USA

    Dennis W. Ross

  2. University of California, Donner Laboratory, 94720, Berkeley, CA, USA

    George Brecher

  3. Institut de Pathologie Cellulaire, Hôpital de Bicêtre, 94270, Bicêtre, France

    Marcel Bessis

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© 1981 Springer-Verlag Berlin Heidelberg

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Orringer, E.P., Roer, M.E.S., Parker, J.C. (1981). Cell Density Profile as a Measure of Erythrocyte Hydration: Therapeutic Alteration of Salt and Water Content in Normal and SS Red Blood Cells. In: Ross, D.W., Brecher, G., Bessis, M. (eds) Automation in Hematology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67756-4_18

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  • DOI: https://doi.org/10.1007/978-3-642-67756-4_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-10225-0

  • Online ISBN: 978-3-642-67756-4

  • eBook Packages: Springer Book Archive

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