Towards Standardization of the Reconstituted Mixed Function Oxidase Systems with Cytochrome P-450

  • M. M. Szutowski
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 4)

Abstract

Reconstituted rabbit pulmonary mixed function oxidase (MFO) system with cytochrome P-450 is composed of NADPH cytochrome P-450 reductase, cytochrome P-450, phospholipid extract and cholate. Factors essential for the reconstitution of the active system include the component concentrations and ratios, preincubation and the ionic strength of the buffer. The rabbit pulmonary MFO system can be separated, purified and reconstituted with good reproducibility. However there are many factors influencing reconstituted activity mainly associated with the reconstitution and reductase purification procedure.

Key words

Cytochrome P-450 Pulmonary MFO system Reconstitution Standardization 

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References

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • M. M. Szutowski
    • 1
  1. 1.Department of Toxicological Chemistry, Institute of BiopharmacyMedical Academy of WarsawWarsawPoland

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