Biotransformation and Spectral Interaction of Xenobiotics with Subcellular Fractions from Drosophila Melanogaster
Several rapid bioassays are in use to detect, by means of mutagenicity, the formation of reactive metabolites from foreign compounds during their metabolism in the organism. Of these bioassays, the fruitfly Drosophila melanogaster was investigated with respect to its capacity to biotransform xenobiotics.
By spectral analysis it was shown that in microsomal preparations of Drosophila the cytochromes P-450 and b5 are present. Microsomes appeared to possess aryl hydrocarbon hydroxylase and epoxide hydratase activities, while post-microsomal supernatants were able to conjugate appropriate compounds with glutathione and phosphate. As yet, glucosyl- and sulfotransferase activities can not be detected. The activities are compared to similar activities in rat liver.
Key wordsXenobiotic-metabolizing enzymes Reactive metabolites Drosophila melanogaster Mutagen test system Rat liver
Unable to display preview. Download preview PDF.
- Baars, A. J., Zijlstra, J. A., Vogel, E., Breimer, D. D.: The occurrence of cytochrome P-450 and aryl hydrocarbon hydroxylase activity in Drosophila melanogaster microsomes, and the importance of this metabolizing capacity for the screening of carcinogenic and mutagenic properties of foreign compounds. Mutation Res. 44, 257–268 (1977)PubMedCrossRefGoogle Scholar
- Montesano, R., Bartsch, H., Tomatis, L. (eds.): Screening tests in chemical carcinogenesis. I.A.R.C. Sci. Publ. No. 12, Lyon, 1976Google Scholar
- Schenkman, J. B., Remmer, H., Estabrook, R. W.: Spectral studies of drug interaction with hepatic microsomal cytochrome (rat). Molec. Pharmacol. 3, 113–123 (1967)Google Scholar
- Vogel, E., Sobels, F. H.: The function of Drosophila in genetic toxicology testing. In: Chemical mutagens. Hollaender, A. (ed.), Vol.4, pp. 93–142. New York: Plenum Publ. Co. 1976Google Scholar
- Yang, R. S. H.: Enzymatic conjugation and insecticide metabolism. In: Insecticide biochemistry and physiology. Wilkinson, C. F. (ed.), pp. 177–225. New York: Plenum Publ. Co. 1976Google Scholar