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Purification and Properties of Membrane-Bound Auxin Receptors in Corn

  • M. A. Venis
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Binding sites in corn coleoptile membranes with high affinity for auxins were first detected by Hertel et al. (1). Subsequently we reported evidence for two kinetic classes of auxin binding sites (2,3) and described their solubilization and partial purification (4). Other workers could distinguish only a single kinetic class of binding sites, either in the membrane-bound (5) or solubilized state (6). On the other hand, Dohrmann et al. (7) have suggested that there are three types of auxin-binding sites, two of which have affinities for 1-naphthylacetic acid (NAA) comparable to those reported by ourselves. The third site, which preferentially binds 2,4-dichlorophenoxyacetic acid (2,4-D) and may be an auxin transport site (8), is only revealed under specialized assay conditions.

Keywords

Auxin Binding Acetone Powder Extract Corn Membrane Supernatant Factor Chromatographic Purification Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • M. A. Venis
    • 1
  1. 1.Shell Biosciences LaboratorySittingbourne Research CentreSittingbourne, KentUK

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