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The Dynamic Process of Assembly of Two-Dimensional Arrays of Macromolecules on Bacteria Cell Walls

  • U. B. Sleytr
  • R. Plohberger
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

So far regular arrays of subunits have been detected on the surface of the cell envelope in a wide range of gram-positive and gram-negative bacteria (see review by Sleytr, 1978). Recent observations indicate that some cyanobacteria may possess similar structures (Kessel, 1978). Although not a universal feature of all prokaryotic cell envelopes, the presence of this regular surface layer (S-layer) may be a more common feature than is generally realized. Electron microscopy, particularly freeze-etching and negative staining methods, has shown that the S-layers have mostly tetragonal or hexagonal symmetry with a center to center spacing between adjacent morphological units from 5 to 20 nm. Chemical analysis of isolated subunits has shown that they are composed mainly of protein or glycoprotein with molecular weights ranging from 50,000 to 200,000 daltons (Sleytr, 1978). In most cases S-layers are built from a Single subunit species, but in some lattices the morphological units seem to be composed of different Polypeptide chains (Plohberger and Sleytr, in preparation).

Keywords

Regular Array Cell Wall Layer Cell Wall Fragment Cell Wall Preparation Scale Mark 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

References

  1. 1.
    Aebi U, Smith PR, Dubochet J, Henry C, Kellenberger E (1973) A study of the structure of the T-layer of Bacillus brevis. J Supramol Struct 1: 498–522PubMedCrossRefGoogle Scholar
  2. 2.
    Beveridge TJ, Murray RGE (1974) Superficial macromolecular arrays on the cell wall of Spirillum putridiconchylium. J Bacteriol 119: 1019–1038PubMedGoogle Scholar
  3. 3.
    Beveridge TJ, Murray RGE (1975) Surface arrays on the cell wall of Spirillum metamorphum. J Bacteriol 124: 1529–1544PubMedGoogle Scholar
  4. 4.
    Beveridge TJ, Murray RGE (1976) Superficial cell wall layers on Spirillum “Ordal” and their in vitro reassembly. Can J Microbiol 22: 567–582PubMedCrossRefGoogle Scholar
  5. 5.
    Brock TD (1978) Thermophilic microorganisms and life at high temperatures, p 116. Springer, Berlin Heidelberg New YorkCrossRefGoogle Scholar
  6. 6.
    Hollaus F, Klaushofer H (1970) Taxonomische Untersuchungen an hoehthermophilen Bazillusstämmen aus Zuckerfabriksäften. Spisy Prir odoved Fak Univ Brne K 47: 99–105Google Scholar
  7. 7.
    Hollaus F, Sleytr UB (1972) On the taxonomy and fine structure of some hyperthermophilic saccharolytic Clostridia. Arch Microbiol 86: 129–146CrossRefGoogle Scholar
  8. 8.
    Kandier O, König H (1978) Chemical composition of the peptidoglycan-free cell walls of methanogenic bacteria. Arch Microbiol 118: 141–152CrossRefGoogle Scholar
  9. 9.
    Kessel M (1978) A unique crystalline wall layer in the cyanobacterium Microcystis marginata. J Ultrastruct Res 62: 203–212PubMedCrossRefGoogle Scholar
  10. 10.
    Matteuzzi D, Hollaus F, Biavati B (1978) Proposal of neotype for Clostridium thermohydro-sulfuricum and the marging of Clostridium tartarivorum with Clostridium thermosaccharolyticum. Int J Syst Bacteriol 28: 528–531CrossRefGoogle Scholar
  11. 11.
    Sleytr UB (1975) Heterologous reattachment of regular arrays of glycoproteins on bacterial surfaces. Nature (London) 257: 400–401CrossRefGoogle Scholar
  12. 12.
    Sleytr UB (1976) Self assembly of the hexagonally and tetragonally arranged subunits of bacterial surface layers and their reattachment to cell walls. J Ultrastrut Res 55: 360–377CrossRefGoogle Scholar
  13. 13.
    Sleytr UB (1978) Regulär arrays of macromolecules on Bacterial cell walls: structure, chemistry, assembly and function. Int Rev Cytol 53: 1–64PubMedCrossRefGoogle Scholar
  14. 14.
    Sleytr UB, Friers GP (1978) Surface Charge and morphogenesis of regular arrays of macromolecules on bacterial cell walls. 9th Int Congr Electron Microsc, vol II, PP 346–347. TorontoGoogle Scholar
  15. 15.
    Sleytr UB, Glauert AM (1975) Analysis of regular arrays of subunits on bacterial surfaces: evidence for a dynamic process of assembly. J Ultrastruct Res 50: 103–116PubMedCrossRefGoogle Scholar
  16. 16.
    Sleytr UB, Glauert AM (1976) Ultrastrueture of the cell walls of two closely related Clostridia that possess different regular arrays of surface subunits. J Bacteriol 126: 869–882PubMedGoogle Scholar

References

  1. 1.
    Aebi U, Smith PR, Dubochet J, Henry C, Kellenberger E (1973) A study of the structure of the T-layer of Bacillus brevis. J Supramol Struct 1: 498–522PubMedCrossRefGoogle Scholar
  2. 2.
    Beveridge TJ, Murray RGE (1974) Superficial macromolecular arrays on the cell wall of Spirillum putridiconchylium. J Bacteriol 119: 1019–1038PubMedGoogle Scholar
  3. 3.
    Beveridge TJ, Murray RGE (1975) Surface arrays on the cell wall of Spirillum metamorphum. J Bacteriol 124: 1529–1544PubMedGoogle Scholar
  4. 4.
    Beveridge TJ, Murray RGE (1976) Superficial cell wall layers on Spirillum “Ordal” and their in vitro reassembly. Can J Microbiol 22: 567–582PubMedCrossRefGoogle Scholar
  5. 5.
    Brock TD (1978) Thermophilic microorganisms and life at high temperatures, p 116. Springer, Berlin Heidelberg New YorkCrossRefGoogle Scholar
  6. 6.
    Hollaus F, Klaushofer H (1970) Taxonomisehe Untersuchungen an hochthermophilen Bazillusstämmen aus Zuckerfabriksäften. Spisy Prir odoved Fak Univ Brne K 47: 99–105Google Scholar
  7. 7.
    Hollaus F, Sleytr UB (1972) On the taxonomy and fine structure of some hyperthermophilic saccharolytic Clostridia. Arch Microbiol 86: 129–146CrossRefGoogle Scholar
  8. 8.
    Kandier O, König H (1978) Chemical composition of the peptidoglycan-free cell walls of methanogenic bacteria. Arch Microbiol 118: 141–152CrossRefGoogle Scholar
  9. 9.
    Kessel M (1978) A unique crystalline wall layer in the cyanobacterium Microcystis marginata. J Ultrastruct Res 62: 203–212PubMedCrossRefGoogle Scholar
  10. 10.
    Matteuzzi D, Hollaus F, Biavati B (1978) Proposal of neotype for Clostridium thermohydro- sulfuricum and the marging of Clostridium tartarivorum with Clostridium thermosaccharolyticum. Int J Syst Bacteriol 28: 528–531CrossRefGoogle Scholar
  11. 11.
    Sleytr UB (1975) Heterologous reattachment of regular arrays of glycoproteins on bacterial surfaces. Nature (London) 257: 400–401CrossRefGoogle Scholar
  12. 12.
    Sleytr UB (1976) Seif assembly of the hexagonally and tetragonally arranged subunits of bacterial surface layers and their reattachment to cell walls. J Ultrastrut Res 55: 360–377CrossRefGoogle Scholar
  13. 13.
    Sleytr UB (1978) Regular arrays of macromolecules on Bacterial cell walls: structure, chemistry, assembly and function. Int Rev Cytol 53: 1–64PubMedCrossRefGoogle Scholar
  14. 14.
    Sleytr UB, Friers GP (1978) Surface Charge and morphogenesis of regular arrays of macromolecules on bacterial cell walls. 9th Int Congr Electron Microsc, vol II, PP 346–347. TorontoGoogle Scholar
  15. 15.
    Sleytr UB, Glauert AM (1975) Analysis of regular arrays of subunits on bacterial surfaces: evidence for a dynamic process of assembly. J Ultrastruct Res 50: 103–116PubMedCrossRefGoogle Scholar
  16. 16.
    Sleytr UB, Glauert AM (1976) Ultrastructure of the cell walls of two closely related Clostridia that possess different regular arrays of surface subunits. J Bacteriol 126: 8692–882Google Scholar
  17. 17.
    Sleytr UB, Thorne KJI (1976) Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum. J. Bacteriol 126: 377–383PubMedGoogle Scholar
  18. 18.
    Thorne KJI (1977) Regularly arranged protein on the surfaces of gram-negative bacteria. Biol Rev 52: 219–234PubMedCrossRefGoogle Scholar
  19. 19.
    Walker PD, Wolf J (1971) The taxonomy of Bacillus stearothermophilus. In: Parker AN, Gould GW, Wolf J (eds) pp 247–263. Spore researeh, Academic Press, London New YorkGoogle Scholar
  20. 20.
    Woese CR, Magrum LJ, Fox GE (1978) Archaebacteria. J Mol Evol 11: 245–252PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • U. B. Sleytr
  • R. Plohberger
    • 1
  1. 1.Zentrum für UltrastrukturforschungUniversität für BodenkulturAustria

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