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Enzyme Activities in Endomyocardial Biopsy Samples from Patients with Cardiomyopathy

  • P. J. Richardson
  • L. Atkinson

Abstract

Endomyocardial biopsy is now an established and safe technique for obtaining samples of fresh ventricular myocardial tissue. Histopathological examination of biopsy samples from patients with suspected cardiomyopathy has improved the accuracy of the clinical diagnosis in approximately 10% of the cases [7], but limitations are still present since, particularly in congestive cardiomyopathy (COCM), the histological findings may not be specific [10], although myocarditis can be excluded. Similarly, in hypertrophic cardiomyopathy (HOCM) histological examination may not confirm the diagnosis as the diseased area may not have been sampled [10]. Highly sensitive enzyme assays already exist for other tissues and it has now been shown that these techniques can be applied to very small myocardial biopsy samples. Comparison of enzyme activities in patients with valvar heart disease and either good or poor left ventricular function has shown that when ventricular function is impaired calcium dependant adenosine triphosphatase (ATPase) activity is strikingly reduced and the activity of lactate dehydrogenase significantly increased [11]. Similarly, in the congestive cardiomyopathies left ventricular tissue showed reduced levels of mitochondrial and myofibrillar enzymes but high levels of lactate dehydrogenase. It has been suggested that defective mitochondrial function is characteristic of both poor left ventricular function, secondary to valvar heart disease, and of congestive cardiomyopathy, and that the increased levels of lactate dehydrogenase reflect a compensatory response [12].

Keywords

Hypertrophic Cardiomyopathy Endomyocardial Biopsy Cytotoxic Therapy Glutamic Oxaloacetic Transaminase Valvar Heart Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bergmeyer HU (1975) Z Klin Chemie klin Biochem 13:508–508Google Scholar
  2. 2.
    Bergmeyer HU, Bernt E, Hess B (1963) In: HU Bergmeyer (ed) Methods of enzymatic analysis. Verlag Chemie, Weinheim, p 736Google Scholar
  3. 3.
    Goodwin JF (1970) Congestive and hypertrophic cardiomyopathy — a decade of study. Lancet I:731–739CrossRefGoogle Scholar
  4. 4.
    Goodwin JF, Oakley CM (1972) The cardiomyopathies. Br Heart J 34:545–552PubMedCrossRefGoogle Scholar
  5. 5.
    Konno S, Sakakibara S (1963) Endomyocardial biopsy. Dis of the Chest 44:345–350CrossRefGoogle Scholar
  6. 6.
    Lowry OH, Roseborough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin-phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  7. 7.
    Mackay EH, Littler WA, Sleight P (1978) Critical assessment of diagnostic value of endomyocardial biopsy. Br Heart J 40:69–78PubMedCrossRefGoogle Scholar
  8. 8.
    Morin Y, Foley AR, Martineau G, Roussel J (1967) Quebec beer drinkers’ cardiomyopathy. Can Med Assoc J 97:881PubMedGoogle Scholar
  9. 9.
    Olsen EGJ (1974) Diagnostic value of the endomyocardial bioptome. Lancet I:658–660CrossRefGoogle Scholar
  10. 10.
    Olsen EGJ (1978) Endomyocardial biopsy (Editorial). Br Heart J 40:95–98PubMedCrossRefGoogle Scholar
  11. 11.
    Peters TJ, Wells G, Brooksby IAB, Jenkins BS, Webb-Peploe MM, Coltart DJ (1976) Enzymic analysis of cardiac biopsy material from patients with valvar heart disease. Lancet I:269–270CrossRefGoogle Scholar
  12. 12.
    Peters TJ, Wells G, Oakley CM, Brooksby IAB, Jenkins BS, Webb-Peploe MM, Coltart DJ (1977) Enzymic analysis of endomyocardial specimens from patients with cardiomyopathies. Br Heart J 39:1333–1339PubMedCrossRefGoogle Scholar
  13. 13.
    Richardson PJ (1974) King’s endomyocardial bioptome. Lancet I:660–661CrossRefGoogle Scholar
  14. 14.
    Richardson PJ, Atkinson Lynne, Jackson G (1978) Myocardial ischaemia and its relationship to coronary arterial disease: Investigation of angina pectoris with normal coronary arteries. In: Carlsson LS, Paoletti R, Weber G (eds) International conference on atherosclerosis. Raven Press, New York, pp 71–74Google Scholar
  15. 15.
    Richardson PJ, Atkinson Lynne, Oram S (1978) Enzyme activities in endomyocardial biopsy samples from patients with cardiomyopathy. (Abstract). Br Heart J 40:456Google Scholar
  16. 16.
    Rosalki SB, Wilkinson JH (1960) Reduction of α-ketobutyrate by human serum. Nature (Lond) 188:1110CrossRefGoogle Scholar
  17. 17.
    Wolfson SK, Williams-Ashman HG (1957) Isocitric and 6-Phosphogluconic dehydrogenase in human blood serum. Proc Soc Exp Biol Med 96:231–234PubMedGoogle Scholar
  18. 18.
    Editorial (1974) Alcoholic cardiomyopathy. Br Medical J 11:731–732Google Scholar
  19. 19.
    (1970) Z Klin Chemie klin Biochem 8:658Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • P. J. Richardson
    • 1
  • L. Atkinson
    • 1
  1. 1.LondonUK

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