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Quantitative Solid Phase Radioimmunoassay of Allergen-Specific IgG

  • R. G. Hamilton
  • N. F. Adkinson
Conference paper

Abstract

Methods for the quantitation of nanogram quantities of allergen-specific IgG in human sera include the antigen-binding technique of Minden and Farr [1], radioimmunoprecipitation assays [2,3], and solid phase radioimmunoassays [4]. The radioimmunoprecipitation (double-antibody) method has been applied to the study of IgG specific for ragweed antigen E [5], bee venom allergens [6], and the penicilloyl hapten [7]. Because of its precision and the possibility of standardization of antibody content, the radioimmunoprecipitation method is considered the procedure of choice for detection of IgG responses to purified allergens. However, when the radioimmunoprecipitation assay is applied to the study of crude allergen mixes, problems arise. These include variable reproducibility in the radioiodination of protein mixes, differential labeling of constituent proteins, and sometimes a rapid decline in immunoreactivity following the labeling procedure. Evaluation of alternative methods which would minimize these problems appears warranted. A solid phase radioimmunoassay for human IgG would be especially attractive since an analogous system, the radioallergosorbent test (RAST) is currently the procedure of choice for measurement of allergen-specific IgE antibody [8,9].

Keywords

Radioimmunoprecipitation Assay Solid Phase Assay Radioallergosorbent Test Honeybee Venom Solid Phase Radioimmunoassay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Minden P, Farr RS (1978) Ammonium sulfate method to measure antigen-binding capacity. In: Weir DM (ed) Handbook of experimental immunology.Google Scholar
  2. 2.
    Skom JH, Talmage DW (1958) Nonprecipitating insulin antibodies. J Clin Invest 37: 787PubMedCrossRefGoogle Scholar
  3. 3.
    Ishizaka K, Ishizaka T, Hornbrook M (1967) Allergen-binding activity of yE, yG and yA antibodies in sera from atopic patients. In vitro measurements of reaginic antibody. J Immunol 98: 490PubMedGoogle Scholar
  4. 4.
    Wide L (1971) Solid phase antigen-antibody systems. In: Kirkham, Hunter (eds) Radioimmunoassay methods. Churchill Livingstone, Edinburgh and LondonGoogle Scholar
  5. 5.
    Yunginger JW, Gleich GJ (1973) Seasonal changes in IgE antibodies and their relationship to IgG antibodies during immunotherapy for ragweed hay fever. J Clin Invest 52: 1268PubMedCrossRefGoogle Scholar
  6. 6.
    Sobotka AK, Valentine MD, Ishizaka K, Lichtenstein LM (1976) Measurement of IgG blocking antibodies: Development and application of a radioimmunoassay. J Immunol 117: 84PubMedGoogle Scholar
  7. 7.
    Gleich GJ, Stankievic RR (1969) Measurement of penicilloyl antibodies by radio- immunoprecipitation. Immunochemistry 6: 85PubMedCrossRefGoogle Scholar
  8. 8.
    Sobotka AK, Adkinson NF, Valentine MD Jr, Lichtenstein LM (1978) Allergy to insect stings. IV. Diagnosis by radioallergosorbent test ( RAST ). J Immunol 121: 2477–2484PubMedGoogle Scholar
  9. 9.
    Adkinson NF Jr (1976) Measurement of total serum Immunglobulin E and allergen- specific immunoglobulin E antibody. In: Rose NR, Friedman H (ed) Manual of clinical immunology. American Society for Microbiology, Washington, DC, p 590Google Scholar
  10. 10.
    Shimizu MM, Wicher K, Reisman R, Arbesman C (1978) A solid phase radioimmunoassay for detection of human antibodies. I. Measurement of IgG antibody to bee venom antigens. J Immunol Methods 19: 317PubMedCrossRefGoogle Scholar
  11. 11.
    Evans R III (1975) In: Evans R III (ed) Advances in diagnosis of allergy: RAST. Proceedings of First North American Conference on RAST, Symposium Specialists, Miami, p 105Google Scholar
  12. 12.
    Sjoquist J (1973) Structure and immunology of Protein A. Contributions to microbiology and immunology, vol I. Karger, Basel, p 83Google Scholar
  13. 13.
    Kronvall G, Williams RC (1969) Differences in Protein A activity among IgG subgroups. J Immunol 103: 828PubMedGoogle Scholar
  14. 14.
    Sobotka AK, Franklin R, Adkinson NF Jr, Valentine MD, Lichtenstein LM (1976) Allergy to insect stings. II. Phospholipase A: The major allergen in honeybee venom. J Allergy Clin Immunol 57: 29Google Scholar
  15. 15.
    Mulligan JJ Jr, Osier AG, Rodriquez E (1966) Weight estimates of rabbit anti- human serum albumin based on antigen-binding capacity. J Immunol 96: 324PubMedGoogle Scholar
  16. 16.
    Schellenberg RR, Adkinson NF Jr (1975) Measurement of absolute amounts of antigen specific human IgE by a radioallergosorbent test ( RAST) elution technique. J Immunol 115: 1577PubMedGoogle Scholar
  17. 17.
    Greenwood FC, Hunter WM, Glover JS (1963) The preparation of 1-131 labeled human growth hormone of high specific radioactivity. Biochem J 89: 114PubMedGoogle Scholar
  18. 18.
    Aalberse RC (1974) Data processing for the radioallergosorbent test RAST. In: Immunoglobulin E, allergens and their interaction. Master’s thesis, N V Rodopi, Amsterdam, p 89Google Scholar
  19. 19.
    Jonsson S, Kronvall G (1974) The use of Protein A-containing Staphylococcus aureus as a solid phase anti-IgG reagent in radioimmunoassays as exemplified in the quantitation of alpha-fetoprotein in normal human serum. Eur J Immunol 4: 29PubMedCrossRefGoogle Scholar
  20. 20.
    Langone JJ, Boyle M, Borsos T (1977) 1-125 Staph Protein A: Applications to the quantitative determination of fluid-phase and cell-bound IgG. J Immunol Method 18: 281–1CrossRefGoogle Scholar
  21. 21.
    Hamilton RG, Sobotka AK, Adkinson NF Jr (1979) Solid phase radioimmunoassay for quantitation of antigen-specific IgG in human sera with 125I-protein A from Staphylococcus aureus. J Immunol 122: 1073–1079PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • R. G. Hamilton
    • 1
    • 2
    • 3
  • N. F. Adkinson
    • 1
    • 2
    • 3
  1. 1.School of MedicineThe Johns Hopkins UniversityBaltimoreUSA
  2. 2.Department of MedicineThe Johns Hopkins UniversityBaltimoreUSA
  3. 3.Division of Clinical ImmunologyThe Johns Hopkins UniversityBaltimoreUSA

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