Quantitative Solid Phase Radioimmunoassay of Allergen-Specific IgG
Methods for the quantitation of nanogram quantities of allergen-specific IgG in human sera include the antigen-binding technique of Minden and Farr , radioimmunoprecipitation assays [2,3], and solid phase radioimmunoassays . The radioimmunoprecipitation (double-antibody) method has been applied to the study of IgG specific for ragweed antigen E , bee venom allergens , and the penicilloyl hapten . Because of its precision and the possibility of standardization of antibody content, the radioimmunoprecipitation method is considered the procedure of choice for detection of IgG responses to purified allergens. However, when the radioimmunoprecipitation assay is applied to the study of crude allergen mixes, problems arise. These include variable reproducibility in the radioiodination of protein mixes, differential labeling of constituent proteins, and sometimes a rapid decline in immunoreactivity following the labeling procedure. Evaluation of alternative methods which would minimize these problems appears warranted. A solid phase radioimmunoassay for human IgG would be especially attractive since an analogous system, the radioallergosorbent test (RAST) is currently the procedure of choice for measurement of allergen-specific IgE antibody [8,9].
KeywordsFiltration Albumin Bromide Assure Borate
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