NMR Studies of Carbonic Anhydrase

  • J. M. Pesando
  • R. K. Gupta
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Hydrolysis of p-nitrophenylacetate to p-nitrophenol and acetate and the reversible hydration of CO2 by the zinc metalloenzyme carbonic anhydrase appear to involve similar catalytic mechanisms (Pocker and Sarkanen, 1978). The essential Zn2+ ion at the active site can be replaced by several divalent cations, but only the Zn2+, Co2+, Mn2+, and Cd2+ enzymes retain catalytic activity. The activity of Zn2+ and Co2+ substituted enzmyes is comparable, while that of the Mn2+ and Cd2+ forms is ~ 10% and ~ 30% that of the native enzyme, respectively (Pocker and Sarkanen, 1978).


Carbonic Anhydrase Nuclear Magnetic Relaxation Paramagnetic Effect Proton Resonance Frequency Human Carbonic Anhydrase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bertini I, Luchinat C, Scozzafava A (1977) Carbon-13 longitudinal relaxation times of acetate ion in the presence of metal-substituted bovine carbonic anhydrases. J Chem Soc, Dalton Trans 1962–1965Google Scholar
  2. Dennard AE, Williams RJP (1966) Transition metal ions as reagents in metallo-enzymes. In: Carlin R (ed) Transition metal chemistry, vol II. Marcel Dekker, New York, pp 115–164Google Scholar
  3. Fabry ME, Koenig SH, Schillinger WE (1970) Nuclear magnetic relaxation dispersion in protein solutions. IV. Proton relaxation at the active site of carbonic anhydrase. J Biol Chem 245: 4256–4262Google Scholar
  4. Gupta RK, Pesando JM (1975) Magnetic resonace study of exchangeable protons in human carbonic anhydrases. J Biol Chem 250: 2630–2634Google Scholar
  5. Jacob GS, Brown RD, Koenig SH (1978) Relaxation of solvent protons by cobalt bovine carbonic anhydrase. Biochem Biophys Res Commun 82: 203–209CrossRefGoogle Scholar
  6. Khalifah, RG (1971) The carbon dioxide hydration activity of carbonic anhydrases. J Biol Chem 246: 2561–2573Google Scholar
  7. Lanir A, Navon G (1974) NMR studies of the two binding sites of acetate ions to manganese ( II) carbonic anhydrase. Biochim Biophys Acta 341: 75–84Google Scholar
  8. Lanir A, Gradsztajn S, Navon G (1973) Proton magnetic relaxation in solutions of manganese carbonic anhydrase. FEBS Lett 30: 351–354CrossRefGoogle Scholar
  9. Liljas A, Kannan KK, Bergsten PC, Waara I, Fridborg K, Strandberg B, Carlbom U, Jarup L, Lovgren S, Petef M (1972) Crystal structure of human carbonic anhydrase C. Nature (London) 235: 131–137Google Scholar
  10. Lindskog S, Coleman JE (1973) The catalytic mechanism of carbonic anhydrase. Proc Natl Acad Sci USA 70: 2505–2508ADSCrossRefGoogle Scholar
  11. Martin RB (1974) Pyrrole hydrogen ionization of imidazole derivatives in metal ion complexes and carbonic anhydrase. Proc Natl Acad Sci USA 71: 4346–4347ADSCrossRefGoogle Scholar
  12. Mildvan AS, Gupta RK (1978) Nuclear relaxation measurements of the geometry of enzyme-bound substrates and analogs. Methods Enzymol 49G: 322–359CrossRefGoogle Scholar
  13. Pesando JM (1975a) Proton magnetic resonance studies of carbonic anhydrase. I. Identification of histidine resonances. Biochemistry 14: 675–680CrossRefGoogle Scholar
  14. Pesando JM (1975b) Proton magnetic resonance studies of carbonic anhydrase. II. Group controlling catalytic activity. Biochemistry 14: 681–688CrossRefGoogle Scholar
  15. Pocker Y, Sarkanen S (1978) Carbonic anhydrase: Structure, catalytic versatility, and inhibition. Adv Enzymol 47: 149–274Google Scholar
  16. Prince RH, Woolley PR (1972) Metal ion function in carbonic anhydrase. Angew Chem Int edn Engl 11: 408–417CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • J. M. Pesando
    • 1
  • R. K. Gupta
    • 2
  1. 1.Sidney Farber Cancer InstituteHarvard Medical SchoolBostonUSA
  2. 2.The Institute for Cancer ResearchThe Fox Chase Cancer CenterPhiladelphiaUSA

Personalised recommendations