Studies of Immune Reactions in Multiple Sclerosis by Active T-Rosette Test, Immunofixation, and Myelinotoxicity of Lymphocytes in the Rabbit Eye

  • H. Link
  • S. Kam-Hansen
  • K. Kristensson
  • M. Laurenzi
  • A. Frydén
  • B. Roström
Conference paper


The rapid development of new or improved techniques in immunology has, during the last few years, considerably increased our understanding of the basis of immune reactions and broadened our knowledge regarding the importance and influence of these reactions in human pathology. However, application of new techniques to functional studies of peripheral blood lymphocytes (PBL) in MS have hitherto not given any conclusive evidence indicating a derangement in cell-mediated immunity outside the CNS. Efforts to study the function of lymphocytes within the CNS have been hampered by the highly cell-consuming techniques in relation to the low lymphocyte numbers obtainable from CSF and by the increased fragility of these cells. The application of technical modifications and microtechniques has, however, enabled studies of CSF lymphocytes compared with PBL regarding B and T lymphocyte distribution and response on mitogen stimulation. Decreased B and elevated T lymphocyte values in CSF compared with blood have thus been reported [11, 26], and CSF lymphocytes have been shown to be poor or nonreactive to the mitogens phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), while PBL proliferate normally on mitogen stimulation [12, 15].


Multiple Sclerosis Multiple Sclerosis Patient Peripheral Blood Lymphocyte Optic Neuritis Aseptic Meningitis 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • H. Link
  • S. Kam-Hansen
  • K. Kristensson
  • M. Laurenzi
  • A. Frydén
  • B. Roström
    • 1
  1. 1.Department of NeurologyUniversity HospitalLinköpingSweden

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