I would like to comment on the radioimmunoassay technique: you know that labeling of the protein with iodine sometimes produces loss of EAE-inducing activity. The molecular conformation is important, and in some cases this may be changed by iodination. Secondly, if you use rabbits for immunization, they sometimes produce a very different kind of antibody. With some antibodies, there is a variety in recognizing the basic protein. Thirdly, you know that some fragments in MS and EAE basic protein may be degraded in various ways. Maybe a different kind of fragment of MBP will be produced and some antibody can actually recognize such a fragment but another serum cannot recognize it. So it depends on the MBP fragments produced.