Discussion

  • H. J. Bauer
  • S. Poser
  • G. Ritter
Conference paper

Abstract

I would like to comment on the radioimmunoassay technique: you know that labeling of the protein with iodine sometimes produces loss of EAE-inducing activity. The molecular conformation is important, and in some cases this may be changed by iodination. Secondly, if you use rabbits for immunization, they sometimes produce a very different kind of antibody. With some antibodies, there is a variety in recognizing the basic protein. Thirdly, you know that some fragments in MS and EAE basic protein may be degraded in various ways. Maybe a different kind of fragment of MBP will be produced and some antibody can actually recognize such a fragment but another serum cannot recognize it. So it depends on the MBP fragments produced.

Keywords

Iodine Electrophoresis Fractionation Tritium 

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • H. J. Bauer
    • 1
  • S. Poser
    • 1
  • G. Ritter
    • 1
  1. 1.Neurologische Klinik und Poliklinik, Neues KlinikumUniversität GöttingenGöttingenGermany

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