Monoclonal Xenogeneic Antibodies to Mouse Leukocyte Antigens: Identification of Macrophage-Specific and Other Differentiation Antigens
The identification and study of cell surface molecules which have specific immune functions and are markers of differentiated white blood cell subpopulations is of great interest in immunology. Antibodies are versatile probes with which to study these molecules. However, it is difficult to obtain highly specific antibodies recognizing individual cell surface molecules, because cell surfaces are complex mosaics of many different immunogenic glycoproteins and glycolipids. A general approach to this problem stems from the experiments of Köhler and Milstein (1). They fused myeloma cells and spleen cells from mice immunized with sheep red blood cells to derive continuous hybrid cell lines secreting antibodies to sheep red blood cells. Such lines can be manipulated in culture so that the multispecific response to a complex immunogen can be resolved into a set of monospecific responses by cloning. Hybrids have been obtained which secrete antibodies to rat major histocompatibility antigens(2), rat cell surface xenoantigens (3), mouse IgD allotypes (4), and mouse H-2K antigens (5).
KeywordsSpleen Cell Antibody Secreting Cell Antigen Titer Major Histocompatibility Antigen Cell Surface Differentiation Antigen
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- 8.Herzenberg, L.A., and Herzenberg, L.A. in Weir, D.M. (Ed). Handbook of Experimental Immunology, 3rd Edit., Blackwell, Oxford p. 12.1 (1978).Google Scholar
- 11.Dawe, C.J., and Potter, M.: Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33, 603 (1957).Google Scholar
- 12.Springer, T., Galfre, G., Secher, D.S., and Milstein, C.: Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol, submitted.Google Scholar
- 13.Stern, P., Willison, K., Lennox, E., Galfre, G., Milstein, C., Secher, D., Ziegler, A., and Springer, T.: Monoclonal antibodies as probes for differentiation and tumor associated antigens: a Forssman specificity on teratocarcinomas and early mouse embryos. Cell submitted.Google Scholar