A Quantitative Disc Radioimmunoassay for Antibodies Directed Against Membrane-Associated Antigens

  • L. A. Manson
  • E. Verastegui-Cerdan
  • R. Sporer
Conference paper

Abstract

The antigens coded for by the major histocompatibility locus are found to exist in cells associated with membranes. As a consequence a number of radioimmunoassays have been developed using whole cells as the immunoadsorbent (1, 2). There are several disadvantages in using whole cells, among them being non-reproducibility of creating monolayers of cells in microtiter plates with adhering cells and the need to centrifuge plates if lymphoid, non-adherent cells are being used.

Keywords

Acetone Albumin Lymphoma Azide 

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References

  1. 1.
    Goldstein, L. T., Klinman, N. R., and Manson, L. A.: Brief Communication: A microtest radioimmunoassay for noncytotoxic tumorspecific antibody to cell-surface antigens. Journal of the National Cancer Institute 51, 1713–1715 (1973).PubMedGoogle Scholar
  2. 2.
    Tax, A. and Manson, L. A.: A radioimmunoassay for antibodies against surface membrane antigens using adhering cells. Journal of Immunological Methods 11, 281–285 (1976).PubMedCrossRefGoogle Scholar
  3. 3.
    Manson, L. A.: Intracellular localization and immunogenic capacities of phenotypic products of mouse histocompatibility genes. In: Biomembranes 8. Manson, L. A. (ed.) Plenum Publishing Corporation, New York: 1976, p. 47–88.Google Scholar
  4. 4.
    Manson, L. A., Foschi, G. V. and Palm, J.: An association of transplantation antigens with microsomal lipoproteins of normal and malignant mouse tissues. Journal of Cellular and Comparative Physiology 61: 109–118 (1963).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • L. A. Manson
  • E. Verastegui-Cerdan
  • R. Sporer

There are no affiliations available

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