A Quantitative Disc Radioimmunoassay for Antibodies Directed Against Membrane-Associated Antigens
The antigens coded for by the major histocompatibility locus are found to exist in cells associated with membranes. As a consequence a number of radioimmunoassays have been developed using whole cells as the immunoadsorbent (1, 2). There are several disadvantages in using whole cells, among them being non-reproducibility of creating monolayers of cells in microtiter plates with adhering cells and the need to centrifuge plates if lymphoid, non-adherent cells are being used.
KeywordsAcetone Albumin Lymphoma Azide
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- 3.Manson, L. A.: Intracellular localization and immunogenic capacities of phenotypic products of mouse histocompatibility genes. In: Biomembranes 8. Manson, L. A. (ed.) Plenum Publishing Corporation, New York: 1976, p. 47–88.Google Scholar