Expression of Thy 1.2 Antigen on Hybrids of B Cells and a T Lymphoma

  • G. M. Iverson
  • R. A. Goldsby
  • L. A. Herzenberg
Conference paper


We and others reported that hybrids of spleen cells and the lymphoma line BW5147 express the Thy1.2 antigen of the splenic donor (1–3). Whether only T cells were the splenic parent of these hybrids could not be concluded from the hybrid phenotypes. We report here that either Ig+ (B) cells or Ig (T and other) cells give hybrids with BW5147 that express the Thy1.2 allele in the genome of the spleen cell whether or not that allele was expressed on the spleen cell parent.


Polyethylene Glycol Spleen Cell Hybrid Population Congenic Strain Hybrid Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Goldsby, R.A., Osborne, B.A., Simpson, E., Herzenberg, L.A.: Hybrid cell lines with T-cell characteristics. Nature, 267, 707–708 (1977)PubMedCrossRefGoogle Scholar
  2. 2.
    Hämmerling, G.J.: T lymphocyte tissue culture lines produced by cell hybridization. Eur. J. Immunol. 7, 743–746 (1977)PubMedCrossRefGoogle Scholar
  3. 3.
    Köhler, G., Lefkovits, I., Elliott, B., Coutinho, A.: Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7, 758–761 (1977)PubMedCrossRefGoogle Scholar
  4. 4.
    Herzenberg, L.A., Herzenberg, L.A.: Analysis and separation using the fluorescence-activated cell sorter (FACS). In: Handbook of Experimental Immunology 3rd Edition, D.M. Weir (ed.). Blackwell Scientific Pub., Oxford, England, Chap. 22 (1978)Google Scholar
  5. 5.
    Pontecorvo, G.: Production of indefinitely multiplying mammalian somatic cell hybrids by polyethylene glycol (PEG) treatment. Somatic Cell Genetics 1, 397–400 (1976)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • G. M. Iverson
  • R. A. Goldsby
  • L. A. Herzenberg

There are no affiliations available

Personalised recommendations