Cellular and Molecular Restrictions of the Lymphocyte Fusion

  • G. Köhler
  • M. J. Shulman
Conference paper


Since 1975 when the first hybridomas secreting antibodies of predetermined specificity were generated (1) the fusion technique has been applied in many laboratories for many different purposes. In the same time several restrictions on cell fusion have become apparent. Some of these restrictions work to the advantage of the experimenter and some to his disadvantage. We wish here to describe how it was possible to exploit some of the benefits and to overcome some of the hindrances that these restrictions imposed on the fusion process.


Spleen Cell Hybrid Line Hybrid Cell Line Mouse Spleen Cell Unimmunized Mouse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Köhler, G. and Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497 (1975).PubMedCrossRefGoogle Scholar
  2. 2.
    Andersson, J., Coutinho, A. and Melchers, F.: Frequencies of mitogen-reactive B cells in the mouse. J. exp. Med. 145: 1520–1530 (1977).PubMedCrossRefGoogle Scholar
  3. 3.
    Potter, M.: Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631–719 (1972).PubMedGoogle Scholar
  4. 4.
    Köhler, G. and Milstein, C.: Derivation of specific antibodyproducing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511–519 (1976).PubMedCrossRefGoogle Scholar
  5. 5.
    Köhler, G., Pearson, T. and Milstein, C.: Fusion of T and B cells. Som. Cell. Genet. 3: 303–312 (1977).CrossRefGoogle Scholar
  6. 6.
    Galfre, G., Howe, S.C., Milstein, C., Butcher, G.W. and Howard, J.C.: Antibodies to major histocompatibility antigens by hybrid cell lines. Nature 266: 550–552 (1977).PubMedCrossRefGoogle Scholar
  7. 7.
    Goldsby R.A. Osborne B.A. and Herzenberg L.A. Hybrid cell lines with T-cell characteristics. Nature 267 707–708 (1977).PubMedCrossRefGoogle Scholar
  8. 8.
    Håmmerling, G.J.: T lymphocyte tissue culture lines produced by cell hybridization. Eur. J. Immunol. 7: 743–746 (1977).PubMedCrossRefGoogle Scholar
  9. 9.
    Hyman, R. and Kelleher, T.: Absence of Thy 1 antigen in L-cell x mouse lymphoma hybrids. Som. Cell. Genet. 1: 335–343 (1975).CrossRefGoogle Scholar
  10. 10.
    Coffino, P., Knowles, B., Nathenson, S. and Scharff, M.D.: Supression of immunoglobulin synthesis by cellular hybridization. Nature New Biol. 231: 87–88 (1971).PubMedCrossRefGoogle Scholar
  11. 11.
    Gilbert, W.: Why genes in pieces. Nature 271: 501 (1978).PubMedCrossRefGoogle Scholar
  12. 12.
    Milstein, C., Adetugbo, K., Cowan, N.J., Köhler, G., Secher, D.S. and Wilde, C. D.: Somatic cell genetics of antibody-secreting cells: studies of clonal diversification and analysis by cell fusion. Cold Spring Harbor Symp. Quant. Biol. 41: 793–803 (1977)PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • G. Köhler
  • M. J. Shulman

There are no affiliations available

Personalised recommendations