Abstract
Development by Milstein et al. (1,2,3) of methods for producing hybrid cell lines synthesizing antibody against antigens of choice should facilitate progress in many areas of biology and immunochemistry. In general it will make possible advances in at least two areas: 1) analysis of the mouse B-cell repertoire and 2) production of monoclonal antibodies to be used as reagents. We will describe here the methods we have used to make progress in both of these areas.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kohler, G., Milstein, C.: Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6, 511–519 (1976).
Kohler, G, and Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495–497 (1975).
Galfre, G., Howe, S.C., Milstein, C., Butcher, G.W., Howard, J.C.: Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266, 550–552 (1977).
Toneqawa, S., Brack, C., Hozumi, N., Matthyssens, G., Schuller, R.: Dynamics of immunoglobulin genes. Immunological Rev. 36, 73–94 (1977).
Klinman, N.R., Sigal, W.H., Metcalf, E.S., Pierce, S.K., Gearhart, P.J.: The interplay of evolution and environment in B-cell diversification. Cold Spring Harbor Symp. Quant. Biol. 41, 165–173 (1977).
Svasti, J., Milstein, C.: The complete amino acid sequence of a mouse κ light chain. Biochem. J. 128, 427–444 (1972).
Margulies, D.H., Kuehl, N.M., Scharff, M.D.: Somatic cell hybridization of mouse myeloma cells. Cell 8, 405–415 (1976).
Littlefield, J.W.: Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145, 709–710 (1964).
Gefter, M.L., Margulies, D.H., Scharff, M.D.: A simple method for polyethylene glycol promoted hybridization of mouse myeloma cells. Somatic Cell Genetics 3, 231–236 (1977).
Klinman, N.R.: The mechanism of antigenic stimulation of primary and secondary clonal precursor cells. J. Exp. Med. 136, 241–260 (1972).
Klinman, N.R., Press, J.L.: The B cell specificity repertoire: Its relationship to definable subpopulations. Transplant. Rev. 24, 49–83 (1975).
Klinman, N.R., Metcalf, E.S., Sigal, N.H.: The functional analysis of B cell muturation at the clonotype level. In: Development of Host Defenses. Dayton, D. (ed.), New York: Academic Press, 1976, pp. 93–106.
Williams, A.F.: Differentiation antigens of the lymphocyte cell surface. Contemp. Topics Mol. Immunol. 6, 83–116 (1977).
Welsh, K.I., Dorval, G., Wigzell, H.: Rapid quantitation of membrane antigens. Nature 254, 67–69 (1975).
Kennett, R.H., Fairbrother, T., Hampshire, B., Bodmer, N.F.: The specificity of naturally ocurring heterophile antibodies in normal rebbit serum. Tissue Antigens 8, 21–28 (1976).
Sato, K., Slesinski, R., Littlefield, J.: Chemical mutagenesis at the phosphoribosyltransferase locus in cultured human lymphoblasts Proc. Natl. Acad. Sci. 69, 1244–1248 (1972).
Potter, M.: Immunoglobulm-producing tumors and myeloma proteins of mice. Physiol. Rev. 52, 631–719 (1972).
Press, J.L., Klinman, 1N.R.: Frequency of hapten-specific B cells in neonatal and adult murine spleens. Europ. J. Immunol. 4, 155–159 (1974).
Goodfellow, P., Barnstable, C., Jones, E., Bodmer, W., Crumpton, M., Snary, D.: Production of specific antisera to human B lymphocytes. Tissue Antigens 7, 105–117 (1976).
Brown, G., Capellaro, D., Greaves, M.: J. Natl. Cane. Instit. 55, 1281–1289 (1975).
Kennett, R.: Unpublished observations, in preparation.
Buck, D., Bodmer, W.F.: The human species antigen on chromosome 11. Birth Defects: Original Article Series 11, 87–89 (1975).
Lampson, L., Levy, R., Grumet, F.C., Ness, D., Pious, D.: Production of in vitro murine antibody to a human histocompatibility alloantigen. Nature 271, 461–462 (1978).
Metzgar, R.S., Mohanakumar, T., Miller, D.S.: Antigens specific for human lymphocytic and myeloid leukemia cells: Detection by nonhuman primate antiserums. Science 178, 986–988 (1972).
Pious, D., Bodmer, J., Bodmer, W.F.: Antigenic expression and cross reactions in HLA variants of lymphoid cell lines. Tissue Antigens 4, 247–256 (1974).
Minowada, J., Ohnuma, T., Moore, G.E.: Brief communication rosette-forming human lymphoid cell lines I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Nat. Cancer Inst. 49 891–895 (1972).
McKusick, V.A., Ruddle, F.H.: The status of the gene map of the human chromosomes. Science 196, 390–405 (1977).
Schwaber, J.: Human lymphocyte-mouse myeloma somatic cell hybrids: selective hybrid formation. Somatic Cell Genetics 3, 295–302, (1977).
Crumpton, M.J., Snary, D.: Preparation and properties of lymphocyte plasma membranes. Contemp. Topics in Mol. Immunol. 3, 27 (1974).
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1979 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Kennett, R.H., Denis, K.A., Tung, A.S., Klinman, N.R. (1979). Hybrid Plasmacytoma Production: Fusions with Adult Spleen Cells, Monoclonal Spleen Fragments, Neonatal Spleen Cells and Human Spleen Cells. In: Melchers, F., Potter, M., Warner, N.L. (eds) Lymphocyte Hybridomas. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67448-8_13
Download citation
DOI: https://doi.org/10.1007/978-3-642-67448-8_13
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-09670-2
Online ISBN: 978-3-642-67448-8
eBook Packages: Springer Book Archive