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Isolierte und charakterisierte Eukaryontengene

  • Peter von Sengbusch

Zusammenfassung

Die in den vorangegangenen Kapiteln beschriebenen Methoden gestatten es, Organisation und Nukleotidsequenzen eukaryotischer Gene zu charakterisieren. Die Hauptschwierigkeit liegt dabei in ihrer Gewinnung. Sobald man einen DNS-Abschnitt mit der gewünschten Nukleotidsequenz in der Hand hat, ist es lediglich eine Frage des Arbeitsaufwandes, diese DNS in genügender Menge zu erzeugen. Das im Kapitel 11 beschriebene Verfahren des Genklonierens gestattet es, DNS beliebiger Herkunft in ausreichenden Mengen zu gewinnen. Die Methoden der Sequenzierung sind mittlerweile ebenfalls äußerst effizient, so daß aufgeklärte Nukleotidsequenzen von Eukaryontengenen in ständig steigender Zahl veröffentlicht werden. Eukaryontengene scheinen interessanter als Prokaryontengene zu sein, denn abgesehen von der Sequenzierung einiger Phagengenome, liegen erst wenige Daten über Sequenzierung bakterieller Gene vor. Oft geht man bei der Sequenzierung eines Gens von mRNS aus spezialisierten Zellen aus.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Peter von Sengbusch
    • 1
  1. 1.Fakultät für BiologieUniversität BielefeldBielefeld 1Deutschland

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